郑燕飞,郭莹

• 1:浙江中医药大学

摘要(Abstract)：

目的优化虎杖中虎杖苷回流提取工艺,并评价其体外抗氧化与抑菌活性。方法采用单因素法考察温度、乙醇体积分数、液料比、提取时间对虎杖提取物中虎杖苷含有量的影响,以Box-Behnken响应面法优化提取工艺;采用DPPH法评价其抗氧化活性;采用牛津杯法测定最小抑菌质量浓度,并绘制抑菌生长曲线。结果虎杖苷的最佳提取工艺:温度77.54℃,乙醇体积分数60%,液料比16.53 m L/g,提取时间1.54 h;虎杖提取物对DPPH自由基具有良好的清除率,且对金黄色葡萄球菌、枯草芽孢杆菌均有抑制作用,并呈剂量依赖性。结论基于响应面法优化虎杖苷所得的提取条件稳定可靠,且虎杖提取物具有一定抗氧化和抑菌作用,对后续的深入研究具有指导意义。

关键词(KeyWords)： 虎杖苷;响应面法;抗氧化;抑菌活性;生长曲线

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(81503206);; 2019国家级大学生创新创业训练计划(771110V017)

作者(Author): 郑燕飞,郭莹

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典（一部）[M].北京：中国医药科技出版社，2010:194.
• [2]Izabela NH,Sylwester S,Sebastian G,et al.Phytochemical diversity in rhizomes of three reynoutria species and their antioxidant activity correlations elucidated by LC-ESI-MS/MSanalysis[J].Molecules,2019,24(6):1136.
• [3]Wu Z,Wang X,Chen M,et al.A study on tissue-specific metabolite variations in polygonum cuspidatum by high-resolution mass spectrometry-based metabolic profiling[J].Molecules,2019,24(6):1058.
• [4]Kuo CH,Chen BY,Liu YC,et al.Optimized ultrasound-assisted extraction of phenolic compounds from polygonum cuspidatum[J].Molecules,2013,19(1):67-77.
• [5]Chen S,Zeng Z,Hu N,et al.Simultaneous optimization of the ultrasound-assisted extraction for phenolic compounds content and antioxidant activity of Lycium ruthenicum Murr[J].Fruit Using Response Surface Methodology,2018,242:1-8.
• [6]潘映桥，黄义云，徐娉，等.响应面法优化酶辅助提取千层金精油的工艺及其体外活性评价[J].中药材，2019,42(3):617-621.
• [7]杜银香，张建伟，胡泽华，等.华中枸骨叶总黄酮超声提取工艺的优化及其抑菌作用[J].中成药，2019,41(9):2200-2203.
• [8]虞夏晖，张旭冉，朱雨晴，等.大黄蒽醌化合物的提取工艺及其体外抑菌活性[J].中华中医药杂志，2018,33(11):5186-5189.
• [9]刘小丽，张伟，符毅文.虎杖中白藜芦醇的超声提取及其抗氧化性研究[J].中成药，2011,33(1):150-153.
• [10]Wang DG,Liu WY,Chen GT.A simple method for the isolation and purification of resveratrol from Polygonum cuspidatum[J].Journal of Pharmaceutical Analysis,2013(4):25-31.
• [11]Zhou J,Zhang L,Li Q,et al.Simultaneous optimization for ultrasound-assisted extraction and antioxidant activity of flavonoids from Sophora flavescens using response surface methodology[J].Molecules,2018,24(1):112.
• [12]袁亚男，陈承瑜，杨滨，等.31种黄酮、酚酸类化合物和10种中药清除DPPH能力考察[J].中国中药杂志，2009,34(13):1695-1700.
• [13]胡婷婷，周畅，胡春萍，等.虎杖苷在ApoE~（-/-）动脉粥样硬化模型小鼠中的抗氧化作用[J].中成药，2016,38(11):2493-2496.
• [14]Yuan ZW,Ouyang P,Gu K,et al.The antibacterial mechanism of oridonin against methicillin-resistant Staphylococcus aureus(MRSA)[J].Pharmaceutical biology,2019,57(1):710-716.
• [15]Jiang KF,Zhao G,Deng GZ,et al.Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κBpathway[J].Acta pharmacologica Sinica,2017,38(2):211-222.
• [16]Uddin Z,Song YH,Curtis-Long MJ,et al.Potent bacterial neuraminidase inhibitors,anthraquinone glucosides from polygonum cuspidatum and their inhibitory mechanism[J].Journal of ethnopharmacology,2016(193):283-292.