杜红彦,李建良,骆煌,闫冉,蔡金英,王蓉

• 1:广东省广州市中医医院

摘要(Abstract)：

目的观察川芎嗪注射液对高眼压大鼠模型视网膜Bcl-2、Bax蛋白表达和视网膜神经节细胞凋亡的影响并探讨其机制。方法应用前房注射羟丙基甲基纤维素的方法建立高眼压大鼠模型,随机分为模型组、川芎嗪组,并设立空白对照组,每组10只。造模成功3 d后开始给药,川芎嗪组给予盐酸川芎嗪注射液7.2 mg/kg腹腔注射。模型组和空白对照组每天用等体积的0.9%氯化钠注射液腹腔注射。每日1次,共2周。观察川芎嗪注射液对高眼压模型大鼠Bcl-2、Bax蛋白表达和视网膜神经节细胞凋亡的影响。结果川芎嗪组视网膜Bcl-2蛋白的表达增高,与模型组和空白对照组比较其间差别有统计学意义(P<0.01);川芎嗪组视网膜Bax蛋白的表达低于模型组,两组间的差异有统计学意义(P<0.01)。川芎嗪组的Bcl-2/Bax比值最高,与模型组和空白对照组比较其间差别有统计学意义(P<0.01)。川芎嗪组视网膜神经节细胞凋亡指数低于模型组,两组间的差异有统计学意义(P<0.01)。结论川芎嗪注射液能够减少高眼压大鼠模型视网膜神经节细胞的凋亡,其机制可能是通过升高视网膜Bcl-2蛋白的表达、降低Bax蛋白的表达、改变Bcl-2/Bax的比值而实现的。

关键词(KeyWords)： 川芎嗪注射液;青光眼;视网膜神经节细胞凋亡;Bcl-2;Bax

Abstract:

Keywords:

基金项目(Foundation): 广东省中医药局科研项目(20141211);; 广东省广州市卫生局科研项目(20142A010003)

作者(Author): 杜红彦,李建良,骆煌,闫冉,蔡金英,王蓉

参考文献(References)：

• [1]Quigley HA,Broman AT.The number of people with glaucoma worldwide in 2010 and 2020[J].Br J Ophthalmol,2006,90:262-267.
• [2]葛坚.我国近五年青光眼临床与基础研究进展[J].中华眼科杂志,2005,56(41):710-716.
• [3]袁援生.青光眼流行病学研究进展[J].昆明医学院学报,2010,31(9):1-3.
• [4]Whitmore AV,Libby RT,John SW.Glaucoma:thinking in new ways-a role for autonomous axonal self-destruction and other compartmentalized processes[J].Prog Retin Eye Res,2005,24(6):639-662.
• [5]Foster PJ.The epidemiology of primary angle closure and associated glaucomatous optic neuropathy[J].Semin Ophthalmol,2002,17(2):50-58.
• [6]Chang EE,Goldberg JL.Glaucoma 2.0:neuroprotection,neuroregeneration,neuroenhancement[J].Ophthalmology,2012,119(5):979-986.
• [7]任培华,冼绍祥,孙敬和,等.养心康对慢性心功能不全兔心室重构的影响[J].中药新药与临床药理,2012,23(1):58-60.
• [8]任培华,张志敏,谭玮璐,等.全真一气汤多糖对慢性心功能不全大鼠TNF-α和IL-6的影响[J].中国中医急症,2014,23(10):1796-1798.
• [9]Wan C,Qian J,Li F,et al.Ultrasound-targeted microbubble destruction enhances polyethylenimine-mediated gene transfection in vitro in human retinal pigment epithelial cells and in vivo in rat retina F[J].Mol Med Rep,2015,12(2):2835-2841.
• [10]Cots D,Bosch A,Chillon M.Helper dependent adenovirus vectors:progress and future prospects[J].Curr Gene Ther,2013,13(5):370-381.
• [11]Puppo A,Cesi G,Marrocco E,et al.Retinal transduction profiles by high-capacity viral vectors[J].Gene Ther,2014,21(10):855-865.
• [12]You Y,Gupta VK,Li JC,et al.0ptic neuropathies:characteristic features and mechanisms of retinal ganglion cell loss[J].Rev Neurosci,2013,24(3):301-321.
• [13]Dan Yi Wang,Arjun Ray,Kathryn Rodgers,et al.Global gene expression changes in rat retinal ganglion cells in experimental glaucoma[J].Invest Ophthalmol Vis Sci,2010,51(8):4084-4095.
• [14]陈伟,彭秀军.视网膜细胞凋亡与bcl-2基因[J].国际眼科杂志,2008,8(6):1236-1238.
• [15]Dongmei He,Baoying Fang.Small hairpin RNA targeting at bcl-2 increases cytarabine-induced apoptosis in raji cells[J].Acta Biochim Biophys Sin,2009,41(2):131-136.
• [16]王雪维.Bcl-2、Bax在青光眼神经节细胞凋亡中的研究进展[J].医学综述,2015,21(22):4077-4079.
• [17]Huang W,Fileta J,Rawe I,et a1.Calpain activation in experimental glaucoma[J].Invest Ophthalmol vis Sci,2010,51(6):3049-3054.
• [18]Rama Rao,Malla,Sreelatha,Gopinath,et al.u PAR and cathepsin B downregulation induces apoptosis by targeting calcineurin A to BAD via Bcl-2 in glioma[J].Journal of neuro-oncology,2012,107(1):69-80.
• [19]Kirti Bhatt,Leping Feng,Navjotsingh Pabla,et al.Effects of targeted Bcl-2 expression in mitochondria or endoplasmic reticulum on renal tubular cell apoptosis[J].AJP-Renal Physiol,2008,294(3):499-507.
• [20]杨雪梅.川芎嗪药理作用研究进展[J].中国生化药物杂志,2010,31(3):215-216.
• [21]Cao J,Miao Q,Zhang J,et al.Inhibitory effect of tetramethylpyrazine on hepatocellular carcinoma:possible role of apoptosis and cell cycle arrest[J].J Biol Regul Homeost Agents,2015,29(2):297-306.