刘佳,孔令凯,孙舒岚,李晓曦,张桂荣,朴浩哲

• 1:辽宁中医药大学
• 2:大连医科大学肿瘤干细胞研究院
• 3:中国医科大学肿瘤医院辽宁省肿瘤医院中心实验室

摘要(Abstract)：

目的观察蟾蜍灵对人胶质瘤细胞系U87MG及U87MG成球细胞增殖和凋亡作用的影响。方法取对数生长期的U87MG细胞,制成细胞悬液,分别向细胞悬液中加入不同浓度的蟾蜍灵,另设DMSO作为对照组,MTT检测细胞存活情况;克隆形成2周后,观察不同浓度蟾蜍灵对细胞增殖的影响;蟾蜍灵处理U87MG细胞12 h和48 h后,流式细胞仪检测细胞凋亡情况;Western blot检测蟾蜍灵处理U87MG细胞48 h后凋亡相关蛋白Cleaved-caspase-3和Cleaved-PARP表达水平;分别用成球培养基和含有蟾蜍灵的成球培养基培养U87MG细胞7 d,显微镜下观察细胞是否成球及其形态、成球体积大小变化和数量变化;成球的U87MG细胞加入不同浓度蟾蜍灵72 h后,PI避光染色1 h,荧光显微镜下观察细胞死亡情况。结果加入蟾蜍灵处理的U87MG细胞生存率较空白对照组和DMSO组明显下降,24 h、48 h和72 h的IC50分别为85 nmol/L、34 nmol/L和22 nmol/L。蟾蜍灵处理后克隆形成数量低于空白对照组。U87MG细胞经蟾蜍灵处理后,细胞凋亡比例增加,并存在药物浓度时间依赖性;凋亡相关蛋白Cleaved-caspase-3和Cleaved-PARP表达量较空白对照组增加;U87MG细胞在成球培养基中培养后可以成球;加蟾蜍灵干预后,细胞成球体积明显比空白对照组小,且数量减少。PI染色观察到随药物浓度增加,细胞球死亡比例增加。结论蟾蜍灵可以抑制U87MG细胞生长和增殖,并能促进其凋亡;蟾蜍灵可以抑制胶质瘤U87MG成球,并能杀伤干细胞。

关键词(KeyWords)： 蟾蜍灵;胶质瘤;凋亡;肿瘤干细胞

Abstract:

Keywords:

基金项目(Foundation): 辽宁省临床能力建设重大项目(LNCCC-B04-2015)

作者(Author): 刘佳,孔令凯,孙舒岚,李晓曦,张桂荣,朴浩哲

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