朱天红,陈本启,赵伟,鲍海琴

• 1:湖北省十堰市人民医院湖北医药学院附属人民医院

摘要(Abstract)：

目的观察茵陈蒿汤对重症急性胰腺炎(SAP)大鼠相关急性肝损伤的作用及p62-keap1-Nrf2信号通路的影响。方法将雄性8周龄SD大鼠30只均分为3组:假手术组、模型组和茵陈蒿汤组。模型组与茵陈蒿汤组用逆行胰胆管注射5%牛磺胆酸钠建立SAP模型,以0.2 m L/min的均匀速度注射到胆管内。假手术组注射等量生理盐水。造模2 h后茵陈蒿汤组以5 g/kg进行灌胃,对照组和模型组给予等量生理盐水。24 h后处死大鼠后进行相关检测。结果假手术组未见病理损害,模型组和茵陈蒿汤组出现不同程度的小叶中心肝细胞坏死,肝细胞颗粒变性,炎性细胞浸润,但茵陈蒿汤组的病理损伤较模型组明显减轻。与假手术组比较,模型组和茵陈蒿汤组大鼠肝脏系数均增高、血清中血清淀粉酶(AMY)、丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(GOT)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)含量均增高,肝组织中p62、keap1和Nrf2蛋白表达均增高;与模型组比较,茵陈蒿汤组大鼠肝脏系数降低,血清中AMY、ALT、GOT、TNF-α和IL-1β含量均降低,肝组织中p62、keap1和Nrf2蛋白表达均降低。结论茵陈蒿汤能通过抑制p62-keap1-Nrf2信号通路从而减轻SAP大鼠急性肝损伤。

关键词(KeyWords)： 重症急性胰腺炎;急性肝损伤;茵陈蒿汤;p62-keap1-Nrf2信号通路;大鼠

Abstract:

Keywords:

基金项目(Foundation): 湖北省卫计委指导项目(WJ2017F079)

作者(Author): 朱天红,陈本启,赵伟,鲍海琴

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