刘玉,谢纬,贾丹,张小瑾,祝庆华,陈生

• 1:广东省深圳市中医院广州中医药大学第四临床医学院

摘要(Abstract)：

目的观察热毒宁注射液对甲型流感病毒感染小鼠肺组织炎症,以及血清和肺组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)表达水平的影响。方法采用滴鼻法建立甲型流感病毒感染小鼠模型。实验设正常对照组、模型组、热毒宁注射液组、利巴韦林注射液组。滴鼻法对小鼠进行流感病毒接种,造模24 h后开始给药5 d,分别于给药后第3、第5日进行取材,计算肺指数,HE染色观察小鼠肺组织病理,ELISA法检测小鼠血清和肺组织TNF-α、IL-1β、IL-6的水平。结果流感病毒感染小鼠后,小鼠肺组织出现炎症改变,肺泡间隔明显增宽,肺指数升高,造模后第5日炎症最为严重。热毒宁注射液组和利巴韦林注射液组肺部炎症有不同程度减轻,热毒宁注射液组作用更明显。流感病毒感染小鼠后,血清和肺泡灌洗液TNF-α、IL-1β、IL-6水平明显增高,在第3日达到高峰;热毒宁注射液和利巴韦林注射液组各时点血清和肺组织TNF-α、IL-1β、IL-6水平均较模型组降低(P <0.05);热毒宁注射液组各时点血清和肺组织TNF-α水平较利巴韦林注射液组降低更明显(P <0.05);治疗第3日,热毒宁注射液组肺组织IL-1β、IL-6水平较利巴韦林注射液组降低更明显(P <0.05)。结论热毒宁注射液可减轻流感病毒感染后的肺部炎症,降低炎症因子的释放。

关键词(KeyWords)： 甲型流感;热毒宁注射液;炎症因子

Abstract:

Keywords:

基金项目(Foundation): 广东省深圳市卫生和计划生育委员会“三名工程”项目(SZSM201812063);; 广东省中医药管理局面上科研项目(20192082)

作者(Author): 刘玉,谢纬,贾丹,张小瑾,祝庆华,陈生

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