芪丹通脉片对缺血/再灌注大鼠心肌细胞凋亡及相关基因Bcl-2与Bax表达的影响Study of the Effect of Qidantongmai Tablet on Reperfusion-induced Myocardial Apoptosis and the Expression of Bcl-2 and Bax in rats
王宗仁,李军昌,王文勇,马静,王文,龙铟
摘要(Abstract)：
目的观察芪丹通脉片对缺血/再灌注大鼠心肌细胞凋亡及相关基因Bcl-2和Bax表达的影响。方法雄性SD大鼠随机分为假手术组、模型组、不同剂量芪丹通脉片组,分组干预7d后,通过结扎冠状动脉左降支40min后再灌注4h成功复制心肌I/RI模型。TUNEL法检测心肌凋亡细胞,免疫组化检测Bcl-2和Bax蛋白的表达。结果不同剂量芪丹通脉片(1.08,3.24g/kg)预处理可抑制I/RI中心肌细胞凋亡,其凋亡指数分别为(14.3±3.8)%和(10.6±2.0)%,与模型组(21.3±4.2)%比较均存在显著差异。模型组Bcl-2平均灰度值为(165.17±9.21);不同剂量芪丹通脉片(1.08,3.24g/kg)预处理后缺血/再灌注心肌组织中Bcl-2的平均灰度值分别为(147.82±19.51)和(118.65±16.31),平均灰度较模型组明显降低;模型组Bax平均灰度值为(139.43±11.70),芪丹通脉片(1.08,3.24g/kg)预处理后Bax-2的平均灰度值分别为(151.67±16.69)和(160.71±19.82),二者的灰度值与模型组比较有降低,但无显著差异。结论芪丹通脉片能抑制缺血/再灌注所致的心肌细胞凋亡,其机制与调节再灌注心肌中Bcl-2、Bax的表达有关。
关键词(KeyWords)： 缺血再灌注;凋亡;Bcl-2;Bax;芪丹通脉片
基金项目(Foundation): 全军医药卫生科研重大临床专项课题(No200619001)
作者(Author): 王宗仁,李军昌,王文勇,马静,王文,龙铟
参考文献(References)：
- 1V an D om burg R T,Sonnenschein K,N ieuwlaat R,et al.Sustained benefit20years after reperfusion therapy in actute therapy in acute m yocardial infarction[J].J A m CollCardiol,2005,46:15～20
- 2Zhao ZQ,M orris CD,Budde JM,et al.Inhibition of m yocardial apop-tosis reduces infarct size and im proves regional contractile dysfunction during reperfusion[J].Cardiovasc R es,2003,59(1):132～142
- 3Y ellon D M,H ausenloy.R ealizing the clinicalpotentialofischem ic pre-conditioning and post conditioning[J].N atClin PractCardiovasc M ed,2005,2(11):568～575
- 4行利,马静,王宗仁,等.芪丹通脉片对大鼠急性心肌缺血损伤的影响[J].第四军医大学学报,2003,24(5):415～417
- 5李晶华,王宗仁,邵中军,等.芪丹通脉片对实验性急性心肌梗死大鼠心肌的保护作用其及分子机制[J].中国临床康复,2004,8(9):1706～1707
- 6朱妙章,袁文俊,吴博威,等.心血管生理学与临床[M].北京:高等教育出版社,2004:633～635
- 7Buja LM,Entrm an M L.M odes ofm yocardialcellinjury and celldeath in ischem ic heart disease[J].Circulation,1998,98:1355～1357
- 8R aphael J,A bedat S,R ivo J,etal.V olatile anesthetic preconditioning attenuates m yocardial apoptosis in rabbits after regional ischem ia and reperfusion via A kt signaling and m odulation of Bcl-2fam ily proteins[J].Pharm acolExp Ther,2006,318(1):186～194
- 9D ong JW,hu H F,Zhu Z,et al.Interm ittent hypoxia attenuates is-chem ia/reperfusion induced apoptosis in cardiac m yocytes via regulating Bcl-2/Bax expression[J].Cell R es,2003,13:385～391
- 10Babu PP,Suzuki G,O no Y,et al.A ttenuation of ischem ia and/or reperfusion injury during m yocardialinfarction using m ild hypotherm ia in rats:an im m unohistochem ical study of Bcl-2,Bax,Bak and TU N EL[J].PatholInt,2004,54(12):896～903
- 11W ang C,N eff D A,K rolikowski JG,et al.The influence of B-cell lym phom a2protein,an antiapoptotic regulator of m itochondrial perm e-ability transition on isoflurane-induced and ischem ic post conditioning in rabbits[J].A nesth A nalg,2006,102(5):1355～1360