邹学红,汪俊,王芳,谢环

• 1:湖北省武汉市汉阳医院

摘要(Abstract)：

目的观察金刚藤颗粒对急性盆腔炎(APID)模型大鼠NLRP3炎症小体通路及免疫功能的影响。方法将雌性SD大鼠105只随机分为假手术组、模型组及金刚藤颗粒低、中、高剂量组,每组均15只,灌胃治疗15 d后,ELISA检测大鼠血清白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)水平;苏木精-伊红(HE)染色观察子宫组织的病理形态;实时定量PCR及Western blotting分别检测NLRP3、ASC、IL-1β、Caspase-1 mRNA及蛋白表达水平。结果 HE染色显示,模型组大鼠子宫内膜上皮细胞脱落坏死,腔壁结构紊乱,全层炎性细胞浸润,有充血水肿;与模型组比较,金刚藤颗粒治疗组症状较模型组有显著改善;与对照组、假手术组比较,模型组血清IL-1β、IL-18水平显著升高(P <0.05),与模型组比较,金刚藤颗粒治疗组IL-1β、IL-18水平显著降低(P <0.05);与对照组比较,模型组NLRP3、ASC、IL-1β、Caspase-1 mRNA水平显著升高(P <0.05),与模型组比较,金刚藤颗粒治疗组NLRP3、ASC、IL-1β、Caspase-1 mRNA水平显著降低(P <0.05);模型组NLRP3、ASC、IL-1β、Caspase-1蛋白表达水平显著高于假手术组(P <0.05),金刚藤颗粒治疗组NLRP3、ASC、IL-1β、Cas-pase-1蛋白表达水平较模型组显著降低(P <0.05)。结论金刚藤颗粒对急性盆腔炎模型大鼠具有显著治疗作用,其可能通过抑制NLRP3炎症小体通路发挥免疫作用。

关键词(KeyWords)： 急性盆腔炎;金刚藤颗粒;NLRP3炎症小体通路;免疫功能;大鼠

Abstract:

Keywords:

基金项目(Foundation): 湖北省自然科学基金项目(2016CFB093)

作者(Author): 邹学红,汪俊,王芳,谢环

参考文献(References)：

• [1]卫雅娴,李振华,解宝江,等.30例女性急性盆腔炎患者的临床分析[J].中华实验和临床感染病杂志:电子版,2016,10(4):422-425.
• [2]龚巍,万莉,刘娜.清热理血汤联合康妇消炎栓治疗老年急性盆腔炎的临床研究[J].中药药理与临床,2016,32(1):202-204.
• [3]王师,王华英,翁跃颂,等.NLRP3炎症小体通路在慢性阻塞性肺病发展中的作用研究[J].浙江医学,2017,39(1):32-36.
• [4]张强,谭超,李阿兴,等.NLRP3炎症小体在失血性休克大鼠肾脏损伤中的作用[J].中国医药导报,2016,13(18):29-32.
• [5]李秀月.金刚藤胶囊治疗慢性盆腔炎疗效观察[J].世界最新医学信息文摘,2016,16(50):110-111.
• [6]李冀红,何延浩,肖云芳,等.康妇炎胶囊对盆腔炎性疾病后遗症模型大鼠免疫功能的影响[J].中国妇产科临床杂志,2017,18(3):230-232.
• [7]Wu D,Guo LY,Lu PS.Effect of acupuncture on inflammatory cytokines in patients with acute pelvic inflammatory disease[J].Acupuncture Research,2018,43(1):57-61.
• [8]Park ST,Lee SW,Min JK,et al.Clinical characteristics of genital chlamydia infection in pelvic inflammatory disease[J].Bmc Womens Health,2017,17(1):5-12.
• [9]Sweet RL.Treatment of acute pelvic inflammatory disease[J].Suppl Int J Gynecol Obstet,2011,2011(1064-7449):13-19.
• [10]Shi ZY,Guo JS,Zuo ZW,et al.Influence of fuke qianjin tablets on NF-κB,IL-6 mRNA in uterine and ovary tissues of acute pelvic inflammatory models of rats[J].Chinese Pharmacological Bulletin,2011,27(12):1771-1772.
• [11]张福梅.金刚藤联合妇科千金片对慢性盆腔炎的疗效及其对患者血清细胞因子IL-6和GM-CSF水平的影响[J].中国妇幼保健,2016,31(13):2606-2608.
• [12]吴大章,苏泰安,代华均,等.金刚藤多糖对大鼠慢性盆腔炎的治疗作用及对血清免疫球蛋白的影响[J].中药药理与临床,2015,31(4):127-129.
• [13]Ye Y,Bajaj M,Yang HC,et al.SGLT-2 inhibition with dapagliflozin reduces the activation of the Nlrp3/ASC inflammasome and attenuates the development of diabetic cardiomyopathy in mice with type 2 diabetes.Further augmentation of the effects with saxagliptin,a DPP4 inhibitor[J].Cardiovasc Drugs Ther,2017,31(2):119-132.
• [14]Yan Chen,Zeng D,Ding L,et al.Three-dimensional poly-(ε-caprolactone)nanofibrous scaffolds directly promote the cardiomyocyte differentiation of murine-induced pluripotent stem cells through Wnt/β-catenin signaling[J].BMC Cell Biology,2015,16(1):1-13.
• [15]师小函,吴本权,石云锋,等.甲型流感病毒H1N1/FM1对小鼠巨噬细胞NLRP3炎症小体信号通路的影响[J].新医学,2017,48(5):302-307.
• [16]Sahoo M,Ceballos-Olvera I,Barrio LD,et al.Role of the inflammasome,IL-1β,and IL-18 in bacterial infections[J].The Scientific World Journal,2011,11(2):2037-2050.
• [17]Abderrazak A,El HK,Bosc E,et al.Inhibition of the inflammasome NLRP3 by arglabin attenuates inflammation,protects pancreaticβ-cells from apoptosis,and prevents type 2diabetes mellitus development in ApoE2.Ki mice on a chronic high-fat diet[J].Journal of Pharmacology&Experimental Therapeutics,2016,357(3):487-494.
• [18]De Nardo D,De Nardo CM,Latz E.New insights into mechanisms controlling the NLRP3 inflammasome and its role in lung disease[J].The American Journal of Pathology,2014,184(1):42-54.
• [19]Liu GQ,Zuo XH,Jiang LN,et al.Inhibitory effect of posthemorrhagic shock mesenteric lymph drainage on the HMGB1 and RAGE in mouse kidney[J].Renal Failure,2016,38(1):131-136.
• [20]赵欢,傅豪,解营利,等.NLRP3炎症小体在大鼠严重烫伤早期心肌内的表达及意义[J].心脏杂志,2016,28(5):508-511.