内关穴位埋线对大鼠心肌梗死后心肌肥大的影响及作用机制Effect and Mechanism of Acupoint Catgut Embedding at Neiguan on Myocardial Hypertrophy after Myocardial Infarction in Rats
平兰芝;黄小楼;胡天俊;姜洪;吴高鑫;
摘要(Abstract):
目的 观察内关埋线对大鼠心肌梗死后心肌肥大的影响,探究其作用机制。方法 采取结扎大鼠左冠状动脉前降支的方法建立心肌梗死动物模型,将其随机分为模型组、内关埋线组、培哚普利组,另设假手术组,其中假手术组只穿线不结扎,每组5只,术后第2天开始干预治疗,干预治疗7 d后,检测大鼠心脏功能,并观察心脏梗死边缘区心肌细胞形态学改变,检测心肌梗死边缘区ANP、m TOR、p-P70S6K、P70S6K、p-4E-BP1、4EBP1的蛋白表达情况。结果 1)与假手术比较,模型组大鼠左室射血分数(LVEF)、左室短轴率(LVFS)明显降低,左室舒张末期内径(LVIDd)、左室收缩末期内径(LVIDs)显著升高,与模型组比较,内关埋线组和培哚普利组LVEF、LVFS显著升高,LVIDd、LVIDs显著降低(P <0.05)。2)与假手术组比较,模型组横截面积显著升高,与模型组比较,内关埋线组和培哚普利组横截面积显著降低(P <0.05)。3)与假手术组比较,模型组ANP蛋白表达显著升高,与模型组比较,内关埋线组和培哚普利组ANP蛋白表达降低(P <0.05)。4)与假手术组比较,模型组p-mTOR/mTOR、p-P70S6K/P70S6K、p-4EBP1/4EBP1蛋白表达水平显著升高(P <0.05);与模型组比较,内关埋线组、培哚普利组p-mTOR/mTOR、p-P70S6K/P70S6K、p-4EBP1/4EBP1蛋白表达水平显著降低(P <0.05)。结论 内关埋线能够改善大鼠心肌梗死后心肌肥大的程度,其机制可能与抑制mTOR/p70S6K/4E-BP1信号通路有关。
关键词(KeyWords): 心肌梗死;心肌肥大;穴位埋线;内关穴;哺乳动物雷帕霉素靶蛋白;mTOR;大鼠
基金项目(Foundation): 贵州中医药大学博士启动项目[贵中医博士启动(2019)138号];贵州中医药大学研究生教育创新计划项目(YCXZR2021011);; 2021年度贵州省中医药民族医药科学技术研究专项课题(QZYY-2021-029)
作者(Authors): 平兰芝;黄小楼;胡天俊;姜洪;吴高鑫;
参考文献(References):
- [1] Luo G,Wang R,Zhou H,et al. ALDOA protects cardiomyocytes against H/R-induced apoptosis and oxidative stress by regulating the VEGF/Notch 1/Jagged 1 pathway[J]. Mol Cell Biochem,2021,476(2):775-783.
- [2] Benjamin EJ,Virani SS,Callaway CW,et al. Heart disease and stroke statistics-2018 update:A report from the American heart association[J]. Circulation,2018,137(12):e67-e492.
- [3] Jenca D,Melenovsk V,Stehlik J,et al. Heart failure after myocardial infarction:incidence and predictors[J]. ESC Heart Fail,2021,8(1):222-237.
- [4] Nakamura M,Sadoshima J. Mechanisms of physiological and pathological cardiac hypertrophy[J]. Nat Rev Cardiol,2018,15(7):387-407.
- [5] Shimizu I,Minamino T. Physiological and pathological cardiac hypertrophy[J]. J Mol Cell Cardiol,2016,97:245-262.
- [6] Li P,Ge J,Li H. Lysine acetyltransferases and lysine deacetylases as targets for cardiovascular disease[J]. Nat Rev Cardiol,2020,17(2):96-115.
- [7] Sciarretta S,Forte M,Frati G,et al. New Insights Into the Role of mTOR Signaling in the Cardiovascular System[J].Circ Res,2018,122(3):489-505.
- [8]陈兴,成传访,方燕龄,等. mTOR信号通路在心肌肥大中的作用[J].中国医药导报,2018,15(7):16-19.
- [9]任晓艳.穴位埋线的源流及其机理探讨[J].中国医药学报,2004,19(12):757-759.
- [10]陈盼碧,陈静,等.穴位埋线法对原发性痛经大鼠神经-内分泌-免疫网络的调控与影响[J].针刺研究,2018,43(1):29-33.
- [11]袁璟,王军蒙,蔡云,等.电针对心肌缺血小鼠心肌组织交感神经相关物质的影响[J].中国针灸,2019,39(5):501-506.
- [12]卢圣锋,袁璟,丁亚娟,等.电针对大鼠缺血心肌组织中炎性物质和交感神经活性物质表达的影响[J].针刺研究,2019,44(5):313-318.
- [13]王一茗,张新昕,束彦页.穴位埋线对缺血再灌注损伤高脂血症合并2型糖尿病大鼠心肌保护作用的机制研究[J].中国全科医学,2017,20(30):3776-3780.
- [14]王一茗,杨晓月,束彦页,等.穴位埋线对心肌缺血再灌注过程自噬相关蛋白表达的影响[J].江苏大学学报:医学版,2017,27(1):1-4.
- [15]梁睿智,刘运珠.近10年针灸治疗冠心病选穴规律探讨[J].中国针灸,2016,36(4):443-447.
- [16]陈纪华,梁睿智,刘运珠.近10年针灸治疗血瘀型胸痹心痛的选穴研究[J].湖南中医杂志,2016,32(11):155-158.
- [17]苏仁强,李伟华,焦杨.内关穴位埋线治疗稳定型心绞痛临床观察[J].上海针灸杂志,2014,33(1):31-33.
- [18]柏树祥,马永华.穴位埋线治疗冠心病[J].针灸临床杂志,2002,18(6):49-50.
- [19] Si LY,Ramalingam A,Ali SS,et al. Roselle attenuates cardiac hypertrophy after myocardial infarction in vivo and in vitro[J]. EXCLI J,2019,18:876-892.
- [20] Yoshida K,Yasujima M,Casley DJ,et al. Effect of chronic neutral endopeptidase inhibition on cardiac hypertrophy after experimental myocardial infarction[J]. Jpn Circ J,1998,62(9):680-686.
- [21] Lee HS,Cho KW,Kim HY,et al. Chamber-specific regulation of atrial natriuretic peptide secretion in cardiac hypertrophy:atrial wall dynamics in the ANP secretion[J]. Pflugers Arch,2020,472(6):639-651.
- [22] Li L,Xu J,He L,et al. The role of autophagy in cardiac hypertrophy[J]. Acta Biochim Biophys Sin,2016,48(6):491-500.
- [23] Laplante M,Sabatini DM. mTOR signaling at a glance[J]. J Cell Sci,2009,122(20):3589-3594.
- [24] Jin J,Hu K,Ye M,et al. Rapamycin reduces podocyte apoptosis and is involved in autophagy and mTOR/P70S6K/4EBP1signaling[J]. Cell Physiol Biochem,2018,48(2):765-772.
- [25] Zhao J,Benakanakere MR,Hosur KB,et al. Mammalian target of rapamycin(mTOR)regulates TLR3 induced cytokines in human oral keratinocytes[J]. Mol Immunol,2010,48(1/3):294-304.
- [26] Sciarretta S,Forte M,Frati G,et al. New insights into the role of mTOR signaling in the cardiovascular system[J]. Circ Res,2018,122(3):489-505.
- [27] Gao XM,Wong G,Wang B,et al. Inhibition of mTOR reduces chronic pressure-overload cardiac hypertrophy and fibrosis[J]. J Hypertens,2006,24(8):1663-1670.
- [28] Shioi T,McMullen JR,Tarnavski O,et al. Rapamycin attenuates load-induced cardiac hypertrophy in mice[J]. Circulation,2003,107(12):1664-1670.