灯盏花素联合三七总苷对急性脑梗死大鼠模型tNOS、iNOS的影响
张文杰,刘冬岩
摘要(Abstract)：
目的探讨中药三七总苷联合灯盏花素对急性脑梗死损伤引起的总一氧化氮合酶(tNOS)活性和诱导型一氧化氮合酶(iNOS)活性的改变及作用机理。方法成年雄性SD大鼠50只,随机分为假手术组、模型组、灯盏花素组、三七总苷组及联合组,应用线栓法制备大鼠急性缺血脑梗死模型,给予联合组灯盏花素8mg/(kg.d)和三七总苷40mg/(kg.d),各稀释至0.5mL,术后2h分别腹腔注射,1次/d,连用3d。测定血清中tNOS含量和iNOS的活性,并进行神经功能缺陷评分。结果联合组能明显降低急性脑梗死大鼠血清中总tNOS含量和iNOS活性。结论灯盏花素联用三七总苷可能通过降低iNOS及tNOS的活性来保护急性脑缺血神经元。
关键词(KeyWords)： 三七总苷;灯盏花素;脑梗死;一氧化氮;一氧化氮合酶
基金项目(Foundation):
作者(Author): 张文杰,刘冬岩
参考文献(References)：
- [1]Stoyanova L L,Lazarov N E.Localization of nitric oxide synthase inrattrigem inal primary afferent neurons using NADPH-diaphorase his-to-chemistry[J].J MoL Histol,2005,36(3):187.
- [2]Holopainen L E Organotypichippocampal slice cultures amodel sys-tem to study basic cellular and molecular mechanisms of neuronal celldeath,neuroprotection,and synaptic plasticity[J].Neurochen Res,2005,30(12):1521.
- [3]Liptin S A,Choi Y B,Pan Z H,et al.A redox-based mechanism for theneuroprotective and neurodestructive effect of nitric oxide and relatednitro-so-com pounds[J].Nature,1993,364(6438):626-32.
- [4]Fassbender K,Fatar M,Ragoschke A,et al.Subacute but not acutegeneration of nitric oxide in focal cerebral ischemia[J].Stroke,2000,31:2208-2211.
- [5]Moro M A,Cardenas A,Hurtado O,et al.Role of nitric oxide after brainischemia[J].Cell Calcium,2004,36:265-275.
- [6]马丽焱,肖培根,梁发权,等,三七总苷对突触体钙摄取功能的影响[J].中国药理学报,1997,18(3):213.
- [7]马丽焱,肖培根,梁发权,等,三七总苷对原代培养大鼠皮质神经细胞的保护作用[J].中国药学杂志,1998,33(3):143.
- [8]田光,石卫东.灯盏花素治疗急性脑梗死126例疗效观察[J].中国实用神经疾病杂志,2008 11(4):71.
- [9]Xia L L,Wei W,Lin H,et al.Experinmental study on protective effectof breviscapine in the liver of diabeticrats[J].Chin pharmacol Bull,2004,20(6):664-668.