厉飞,裘涛,胡娅娜,陶水良

• 1:浙江中医药大学
• 2:浙江中医药大学附属第一医院
• 3:浙江省金华市中医院
• 4:浙江中医药大学基础医学院

摘要(Abstract)：

目的探讨郁金提取物对神经病理性疼痛模型(CCI)大鼠痛觉行为及脑源性神经营养因子(BDNF)表达的影响。方法建立CCI大鼠模型,将SD大鼠分为正常组、模型组、郁金提取物低、中、高剂量组,加巴喷汀组和溶剂组,对各组大鼠进行机械缩足阈值(PMWT)测定,用ELISA法测定大鼠血清中BDNF的表达。结果(1)与正常组相比,造模后大鼠PMWT显著下降(P<0.05),说明神经病理性模型造模成功;与溶剂组相比,郁金提取物高剂量组和加巴喷汀组显著上调CCI模型大鼠PMWT(P<0.05)。(2)造模后大鼠血液内BDNF表达均较正常组显著增加(P<0.05);与溶剂组相比,郁金提取物中、高剂量组和加巴喷汀组能显著降低血液中BDNF的表达(P<0.05)。郁金提取物高剂量组、加巴喷汀组和正常组BDNF表达无显著差异。结论郁金提取物可能通过降低CCI模型大鼠血清BDNF的表达而发挥抗神经病理性疼痛的作用。

关键词(KeyWords)： 郁金提取物;神经病理性疼痛;BDNF

Abstract:

Keywords:

基金项目(Foundation): 浙江省中医药管理局科研课题(2013ZB031)

作者(Author): 厉飞,裘涛,胡娅娜,陶水良

参考文献(References)：

• [1]Vranken JH.Elucidation of pathophysiology and treatment of neuropathic pain[J].Cent Nerv Syst Agents Med Chem,2012,12(4):304-314.
• [2]Siniscalco D,Giordano C,Rossi F,et al.Role of neurotrophins in neuropathic pain.Role of neurotrophins in neuropathic pain[J].Curr Neuropharmacol,2011,9(4):523-529.
• [3]Beggs S,Salter MW.The known knowns of microglia-neuronal signalling in neuropathic pain[J].Neurosci Lett,2013,17(557):37-42.
• [4]Toulme E,Khakh BS.Imaging P2X4 receptor lateral mobility in microglia:regulation by calcium and p38 MAPK[J].J Biol Chem,2012,287(18):14734-14748.
• [5]Trang T,Beggs S,Salter MW.Brain-derived neurotrophic fact or from microglia:a molecular substrate for neuropathic pain[J].Neuron Glia Biol,2011,7(1):99-108.
• [6]WU Lin hua,DU Xia,LIU Hong mei.β-Elemene from volatile oil of Curcuma kwangsiensis by supercritieal fluid extraction[J].Chinese Traditional and Herbal Drugs,2006,37(3):360-370.
• [7]Bennett GJ,Xie YK.A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man[J].Pain,1988,33(1):87-107.
• [8]龚敏操,许俊杰,陈眉.郁金提取物对小鼠的镇痛作用[J].浙江中医药大学学报,2010,34(2):266-269.
• [9]Santos AR,Comprido D,Duarte CB.Regulation of local translation at the synapse by BDNF[J].Prog Neurobiol,2010,92(4):505-516.
• [10]Obata K,Noguchi K.BDNF in sensory neurons and chronic pain[J].Neurosci Res,2006,55(1):1-10.
• [11]Toyomitsu E,Tsuda M,Yamashita T,et al.CCL2promotes P2X4 receptor trafficking to the cell surface of microglia[J].Purinergic Signal,2012,8(2):301-310.
• [12]Biber K,Tsuda M,Tozaki-Saitoh H,et al.Neuronal CCL21up-regulates microglia P2X4 expression and initiates neuropathic pain development[J].EMBO J,2011,30(9):1864-1873.
• [13]Ferrini F,De Koninck Y.Microglia control neuronal network excitability via BDNF signalling[J].Neural Plast,2013,2013:429815.
• [14]Chen HS,Zhou ZH,Li M,et al.Contribution of brain-derived neurotrophic factor to mechanical hyperal gesia induced by ventral root transection in rats[J].Neuroreport,2013,24(4):167-170.
• [15]Constandil L,Aguilera R,Goich M,et al.Involvement of spinal cord BDNF in the generation and maintenance of chronic neuropathic pain in rats[J].Brain Res Bull,2011,86(5-6):454-459.