张典,张茜,许悦,陈烯琳,秦露雪,郭长青

• 1:北京中医药大学

摘要(Abstract)：

目的 观察针刀干预对膝骨关节炎（KOA）兔软骨血管分布及CD34、CD105、血管内皮生长因子（VEGF）表达的影响，探讨其治疗KOA的机制。方法 采用改良后Videman左后肢伸直位固定法制备KOA兔模型，将28只新西兰雄兔随机等分为空白组、模型组、电针组和针刀组，每组7只。空白组不予处理，干预3周后，取软骨-软骨下骨复合体组织块进行苏木素-伊红（HE）染色观察组织学变化；免疫组织化学法观察CD34、CD105表达来计算微血管密度（MVD）；酶联免疫吸附法（ELISA）检测血清及滑液VEGF蛋白表达水平。结果HE染色：软骨下骨血管异常生成，突破潮线到达非钙化软骨层。免疫组化：与空白组相比，其余3组兔CD34、CD105蛋白表达及MVD值均显著上升（P<0.01）；与模型组相比，电针组和针刀组均显著降低（P<0.01），针刀组显著低于电针组（P <0.05）。ELISA检测：与空白组相比，模型组血清及滑液中VEGF表达水平显著增加（P <0.01）；与模型组相比，电针组和针刀组显著减少（P<0.01），针刀组显著低于电针组（P<0.05）。结论 针刀干预可以通过“调筋”恢复关节良性应力环境，有效抑制KOA兔软骨血管异常生成，发挥软骨保护作用从而实现“治骨”。

关键词(KeyWords)： 膝骨关节炎;针刀;血管生成;MVD;CD34;CD105;VEGF;兔

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目（82074523）

作者(Author): 张典,张茜,许悦,陈烯琳,秦露雪,郭长青

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