牟芳芳,于永娟,张泽安,陆萍萍,邵水金

• 1:上海中医药大学

摘要(Abstract)：

目的应用体外培养的大鼠脑皮层星形胶质细胞,观察银杏叶提取物(EGb761)对氧糖剥夺再复氧导致细胞损伤的影响,并探讨其作用机制。方法以连二亚硫酸钠和无糖DMEM液造成化学性氧糖剥夺(OGD)模型模拟体内脑缺血再灌注损伤。利用WST-1法检测细胞活性、细胞上清液中乳酸脱氢酶(LDH)检测细胞损伤程度、GFAP染色及Hoechst 33258染色观察细胞形态变化和凋亡,免疫蛋白印迹法测定bcl-2、bax、cas-pase-3蛋白的表达。结果 OGD处理90 min再复氧24 h能使细胞存活率明显下降,LDH漏出量增高,细胞形态损伤性改变、凋亡细胞增多,并能下调bcl-2/bax比值和上调caspase-3表达。而给予EGb761可逆转上述变化,减轻细胞损伤。结论 EGb761对OGD/R诱导的星形胶质细胞损伤具有保护作用,其作用机制可能与调整bcl-2/bax比值、抑制caspase-3激活对抗细胞凋亡有关。

关键词(KeyWords)： 银杏叶提取物;星形胶质细胞;凋亡;bcl-2;bax;caspase-3

Abstract:

Keywords:

基金项目(Foundation): 上海市教育委员会科研创新项目(09YZ123);; 上海市教育委员会重点学科分课题(J50301);; 上海中医药大学名师传承研究工程项目(2009120)

作者(Author): 牟芳芳,于永娟,张泽安,陆萍萍,邵水金

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