丹参酮ⅡA对脓毒症大鼠外周血CD4~+T细胞调节的作用研究Effect of Tanshinone Ⅱ A on Peripheral Blood CD4~+T Cells in Rats with Sepsis
彭戬,程光,马军,王南下
摘要(Abstract)：
目的探讨丹参酮ⅡA对脓毒症大鼠外周血CD4+T细胞的作用。方法采用盲肠结扎穿孔术制备脓毒症大鼠模型(CLP),给予丹参酮ⅡA(TanⅡA)处理,分别于治疗前及治疗后24、48、72 h各时间点取静脉血,流式细胞仪检测全血细胞中辅助性T细胞(Th1、Th2、Th17);采用酶联免疫吸附法(ELISA)测定血中白细胞介素-1(IL-1)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)及肿瘤坏死因子-α(TNF-α)水平。结果 CLP组大鼠血清TNF-α、IL-17、IL-1水平和Th17比例明显高于同期Sham组(P<0.05),而TanⅡA各亚组血清TNF-α、IL-17、IL-1水平和Th17比例均低于CLP同期各亚组(P<0.05);CLP组大鼠血清IL-10水平和Th1/Th2比值明显低于Sham组(P<0.05),而TanⅡA各亚组血清IL-10水平和Th1/Th2比值高于CLP同期各亚组(P<0.05)。结论丹参酮ⅡA可能通过作用于脓毒症大鼠的外周血CD4+T细胞发挥调节免疫、改善炎症反应的作用。
关键词(KeyWords)： 丹参酮ⅡA;脓毒症;CD4+T细胞
基金项目(Foundation): 国家自然科学基金(30500657);; 湖北省自然科学基金(2011CDB196)
作者(Author): 彭戬,程光,马军,王南下
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