杨梦霞,毛昀,芦殿荣,朱世杰,周磊

• 1:北京中医药大学
• 2:中国中医科学院望京医院
• 3:湖南中医药大学第二附属医院

摘要(Abstract)：

目的 从网络药理学角度初步探究阴阳双补方治疗骨转移癌痛的潜在分子作用机制。方法 中药数据库筛阴阳双补方主要化合物，并利用Swiss Target Prediction数据库检索化合物对应靶点，通过GeneCards检索骨转移和癌痛两种疾病基因。通过韦恩分析将化合物靶点和两种疾病基因进行映射，得到潜在治疗靶点；利用Cytoscape3.8.2软件绘制多层次相互作用的“中药-化合物-潜在治疗靶点”网络图，并利用DAVID平台对潜在治疗靶点进行富集分析。结果 阴阳双补方主要化合物有88个，含673个药物靶点。骨转移癌疾病基因有483个、癌痛基因有858个，与药物靶点映射后得到94个潜在治疗靶点。阴阳双补方治疗骨转移癌痛的关键靶点为VEGFA、STAT3、ErbB2及AKT1等。通过富集分析发现，阴阳双补方治疗骨转移癌痛是通过调控PI3KAkt、FoxO、VEGF及mTOR等通路发挥抗炎、镇痛、调节神经系统的作用。结论 通过中药网络药理学发现“阴阳双补方”治疗骨转移癌痛的作用机制复杂，涉及多成分、多靶点和多通路，可能与抗炎、镇痛、调节神经系统等相关。

关键词(KeyWords)： 肿瘤;骨转移;疼痛;网络药理;机制探讨;阴阳双补方

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(81973640);; 专利：一种治疗骨转移癌性躯体痛的中药组合物及其制备方法和应用(202110174580.6);; 中国中医科学院望京医院院级课题(WJYY2020-26)

作者(Author): 杨梦霞,毛昀,芦殿荣,朱世杰,周磊

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