加味香连丸对急性放射性肠炎大鼠NF-κB信号通路的影响Effect of Jiawei Xianglian Pills on NF-κB Signaling Pathway in Rats with Acute Radiation Enteritis
苏景阳,傅越,王梦蕾,严江,王珏,林胜友
摘要(Abstract)：
目的 探究加味香连丸对急性放射性肠炎大鼠核转录因子-κB(NF-κB)信号通路的影响。方法 将40只SPF级SD大鼠随机分为正常组、模型组、加味香连丸组、思密达组。对除正常组外的3组大鼠予直线加速器6-MV的X射线单次全腹部照射，总剂量12 Gy，剂量率200 cGy/min，建立急性放射性肠炎模型。照射后12 h开始灌胃，观察各组大鼠形态学、体质量变化；利用光学显微镜下观察及图像分析软件对比各组肠组织病理改变，并用ELISA法测定肠组织Toll样受体4(TLR-4)、NF-κB p65、白细胞介素（IL）-6、肿瘤坏死因子-α(TNF-α)含量。结果 模型大鼠均出现急性放射性肠炎症状，小肠组织不同程度地出血、坏死。加味香连丸组TLR-4、NF-κB p65及相关炎症因子IL-6、TNF-α含量与正常组比较未见明显差异（P> 0.05）。结论 加味香连丸对急性放射性肠炎大鼠NF-κB信号通路存在影响，能改善相关炎症指标，阻止炎症信号放大，对肠道黏膜起到一定的保护作用。
关键词(KeyWords)： 急性放射性肠炎;加味香连丸;NF-κB信号通路;大鼠
基金项目(Foundation): 国家自然科学基金项目（81904197）;; 浙江省2018年度重点研发计划项目（2018C03025）;; 浙江省林胜友名老中医专家传承工作室（浙中医药[2020]8号,GZS202002）
作者(Author): 苏景阳,傅越,王梦蕾,严江,王珏,林胜友
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