张志敏,任培华,陈康,张大鹏

• 1:广州医学院第一附属医院

摘要(Abstract)：

目的观察全真一气汤总多糖对免疫抑制小鼠血清IL-6和TNF-α的影响。方法取SPF级KM小鼠24只随机分为正常对照组、模型组和全真一气汤总多糖组,分别给予生理盐水和全真一气汤总多糖灌胃,共8d。在第7日给模型组、全真一气汤总多糖组小鼠腹腔注射环磷酰胺。采用酶免法检测各组IL-6和TNF-α。结果模型组血清IL-6和TNF-α水平明显降低,与正常对照组比较,具有显著差异,而全真一气汤多糖组IL-6和TNF-α水平与模型组比较明显升高,具有显著差异。全真一气汤多糖组与正常对照组比较差异无统计学意义。结论全真一气汤多糖可升高免疫抑制小鼠血清的IL-6和TNF-α水平,表明全真一气汤多糖具有刺激免疫抑制小鼠机体的细胞因子分泌,调节免疫功能的作用。

关键词(KeyWords)： 全真一气汤总多糖;免疫抑制;IL-6;TNF-α

Abstract:

Keywords:

基金项目(Foundation): 广东省社会发展领域科技计划项目([2010]-1096)

作者(Author): 张志敏,任培华,陈康,张大鹏

参考文献(References)：

• [1]张志敏.全真一气汤配合西药治疗顽固性心力衰竭疗效观察[J].中国中医急症,2005,14(8):714,767
• [2]张志敏.全真一气汤治疗慢性阻塞性肺气肿78例[J].江西中医药,2005,36(7):27-28
• [3]Petrovic,Rackov L.Cytokines in rheumatoid arthritis andosteoar-thro-sis[J].Med Pregl,2005,58:245-251.
• [4]Ravaglia G,Forti P,Maioli F,et al.Associations of the 174g/cinter-leukin-6 gene promoter polymorphism with blood interleukin-6 andmortality in the elderly[J].Biogerontology,2005,6:415-423.
• [5]Boniface K,Lecron J C,Bernard F X,et al.Keratinocytes as targets forinterleukin-10-related cytokines:a putative role in the pathogenesis ofpsoriasis[J].Eur Cytokine Netw,2005,16:309-319.
• [6]Luppi P,Licata A,Haluszczak C,et al.Analysis of TCR Vbeta reper-toire and cytokine gene expression in patients with idiopathic dilatedcardio-myopathy[J].J Autoimmun,2001,16(1):3-13.
• [7]Chen J W,Chen Y H,Lin S J.Long-term exposure to oxidized lowden-sity lipoprotein enhances tumor necrosis factor-alpha stimulatedendo the lial adhesiveness of monocytes by activating superoxide gen-eration and redox-sensitive pathways[J].Free Radic Biol Med,2006,40:817-826.
• [8]张丰,曹苇,王玮,等.免疫抑制大鼠模型感染后TNF-α和IL-6的基因表达[J].苏州大学学报:医学版,2005,25(1):16-19