郝霁萍,高宇勤,贺少辉,赵国平

• 1:陕西省西安市第九医院
• 2:暨南大学医学院

摘要(Abstract)：

目的探究芍药苷预处理通过激活过氧化物酶体增殖物激活受体(PPARα)信号通路对大鼠在体心肌缺血再灌注损伤发挥的干预作用。方法采用结扎SD大鼠心脏LDA的方法建立在体大鼠心肌缺血再灌注损伤模型,利用0.5%Evan’s蓝灌注心肌及1%TTC磷酸缓冲液孵育心肌来观察心肌坏死利用HE染色观察心肌损伤程度,利用酶联免疫吸附法观察大鼠血清中肿瘤坏死因子(TNF-α)、细胞黏附因子(ICAM-1)的含量,蛋白印迹(Western blot)技术检测芍药苷预处理对PPARα的影响。结果芍药苷药物预处理组与缺血再灌注组比较,心肌梗死面积明显减小(P<0.01);芍药苷预处理能促进PPARα蛋白的表达,减少血清中TNF-α、ICAM-1的含量。结论芍药苷预处理能减轻大鼠在体心肌缺血再灌注损伤,可能通过增加PPARα蛋白的表达,抑制TNF-α、ICAM-1分泌,减少炎症反应,进而发挥保护作用。

关键词(KeyWords)： 芍药苷;心肌缺血再灌注损伤;过氧化物酶体增殖物激活受体

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81173189);; 西安市卫生局科技项目;; 西安市科技项目资助(SF1416)

作者(Author): 郝霁萍,高宇勤,贺少辉,赵国平

参考文献(References)：

• [1]Hecker M1,Behnk A,Morty RE,et al.PPAR-αactivation reduced LPS-induced inflammation in alveolar epithelial cells[J].Exp Lung Res,2015,7:1-11.
• [2]Yue TL,Bao W,Jucker BM,et al.Activation of peroxisome proliferator-activated receptor-alpha protects the heart from ischemia/reperfusion injury[J].Circulation,2003,108:2393-2399.
• [3]Marx N,Sukhova GK,Collins T,et al.PPARalpha activators inhibit cytokine-induced vascular cell adhesion molecule-1expression in human endothelial cells[J].Circulation,1999,99:3125-3131.
• [4]Hou G,Yin Y,Han D,et al.Rosiglitazone attenuates the metalloprotease/anti-metalloprotease imbalance in emphysema induced by cigarette smoke:involvement of extracellular signal-regulated kinase and NFκB signaling[J].Int J Chron Obstruct Pulmon Dis,2015,10:715-24.
• [5]Xiaofei Li,Zhibin Wen,Xiaofan He,et al.Effects of cinnamic acid on expression of tissue factor induced by TNF-αin endothelial cells and its mechanisms[J].J Chin Med Assoc,2006,69(5):207-212.
• [6]Aliaksandr A Bulhak,Christian Jung,Claes-Go,et al.PPARαactivation protects the type 2 diabetic myocardium against ischemia-reperfusion injury:involve ment of the PI3-Kinase/Akt and NO pathway[J].Am J Physiol Heart Circ Physiol,2009,296:H719-H727.