柯淑红,郑承红,曹萍

• 1:湖北省武汉市第一医院

摘要(Abstract)：

目的观察心肌细胞间质纤维化、转化生长因子β1(TGF-β1)基因在糖尿病大鼠心肌组织的表达及糖脂安的干预作用。方法尾静脉注射链脲佐菌素(STZ)并高糖高脂饲料喂养诱导2型糖尿病大鼠模型;大鼠分为中药组(糖脂安组)、模型组、正常对照组。治疗8周后,行Masson染色观察心肌胶原分布并测定心肌胶原含量,行逆转录-聚合酶链式反应(RT-PCR),检测心肌组织中TGF-β1mRNA表达水平。结果与正常组比较,模型组心肌胶原蛋白含量和TGF-β1mRNA的表达均明显升高(P<0.01);中药组上述指标均显著低于模型组。结论 TGF-β1表达增高导致细胞间质降解减少并生成增多,是心肌间质纤维化原因,而糖脂安可以干预TGF-β1mRNA表达,预防心肌组织的纤维化。

关键词(KeyWords)： 糖脂安;2型糖尿病;心肌;转化生长因子β1

Abstract:

Keywords:

基金项目(Foundation): 湖北省武汉市卫生科研基金项目(2007-43)

作者(Author): 柯淑红,郑承红,曹萍

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