温秀梅,蔡丹莉,严茂祥,何琼笑,王斌

• 1:浙江中医药大学附属第三医院
• 2:浙江省中医院

摘要(Abstract)：

目的观察大黄素对重症急性胰腺炎(SAP)肺损伤大鼠肺组织FGL2凝血酶原酶的影响。方法采用逆行胰胆管注射牛磺胆酸建立SAP肺损伤大鼠模型,分别以大黄素高(40 mg/kg)、中(20 mg/kg)、低剂量(10 mg/kg)进行干预,在造模3、6、12 h 3个时相点分批处死大鼠,采用qRT-PCR检测大鼠肺组织FGL2、TNF-αmRNA表达,免疫组化技术检测肺组织中FGL2蛋白表达,ELISA法检测肺组织TNF-α及TXB2、6-Keto-PGF1α水平。结果模型组大鼠3、6、12 h 3个时相点肺组织FGL2、TNF-αmRNA和蛋白表达,TXB2水平及TXB2/6-Keto-PGF1α比值较假手术组同一时相点明显增高(P <0.05或P <0.01),应用大黄素高、中、低剂量干预后,肺组织FGL2、TNF-αmRNA和蛋白表达,TXB2水平及TXB2/6-Keto-PGF1α比值较模型组同一时相点显著下降(P <0.05或P <0.01)。结论重症急性胰腺炎肺损伤时大黄素可以通过下调FGL2凝血酶原酶减轻胰腺炎肺损伤程度,这可能是大黄素抗SAP肺损伤的重要作用机制。

关键词(KeyWords)： 重症急性胰腺炎;肺损伤;大黄素;FGL2凝血酶原酶;大鼠

Abstract:

Keywords:

基金项目(Foundation): 浙江省中医药科研基金计划资助项目(2016ZB064);; 浙江中医药大学附属第三医院科研计划资助项目(2017ZS02-01)

作者(Author): 温秀梅,蔡丹莉,严茂祥,何琼笑,王斌

参考文献(References)：

• [1]曹均强，汤礼军.全身炎症反应综合征在急性胰腺炎肺损伤中的研究进展[J].中华消化外科杂志，2015,14(11):975-979.
• [2] Xing Mao Wu,Kai Qiang Ji,Hai Yuan Wang,et al. Micro RNA-339-3p alleviates inflammation and edema and suppresses pulmonary microvascular endothelial cell apoptosis in mice with severe acute pancreatitis-associated acute lung injury by regulating Anxa3 via the Akt/mTOR signaling pathway[J]. Journal of Celluar Biochemistry,2018,119(8):6704-6714.
• [3] Zhao Z,Wang L,Yang C,et al. Soluble FGL2 induced by tumor necrosis factor-αand interferon-γin CD4+T cells through MAPK pathway in human renal allograft acute rejection[J]. Journal of Surgical Research,2013,184(2):1114-1122.
• [4] Jia P,Wang J,Wang L,et al. TNF-αupregulates Fgl2 expression in rat myocardial ischemia/reperfusion injury[J]. Microcirculation,2013,20(6):524-533.
• [5] Ye X,Ding J,Chen Y,et al. Adenovirus-mediated artificial miRNA targetting fibrinogen-like protein 2 attenuates the severity of acute pancreatitis in mice[J]. Bioscience Reports,2017,37(6):BSR20170964.
• [6] Wu L,Cai B,Zheng S,et al. Effect of emodin on endoplasmic reticulum stress in rats with severe acute pancreatitis[J]. Inflammation,2013,36(5):1020-1029.
• [7] Li J,Zhou R,Bie BB,et al. Emodin and baicalein inhibit sodium taurocholate-induced vacuole formation in pancreatic acinar cells[J]. World J Gastroenterol,2018,24(1):35-45.
• [8]石占利，李国辉，孙静，等.大黄素对重症急性胰腺炎并发急性肺损伤大鼠的干预作用[J].中华危重症医学杂志：电子版，2015,8(3):143-149.
• [9]蔡丹莉，高凯，陈芝芸，等.大黄素对重症急性胰腺炎肺损伤大鼠胰腺及肺组织Toll样受体4和9表达的影响[J].浙江中医杂志，2018,53(12):914-915.
• [10] Dombernowsky T,Kristensen M?，Rysgaard S,et al. Risk factors for and impact of respiratory failure on mortality in the early phase of acute pancreatitis[J]. Pancreatology,2016,16(5):756-760.
• [11] Akbal E,Demirci S,Ko?ak E,et al. Alterations of platelet function and coagulation parameters during acute pancreatitis[J]. Blood Coagul Fibrinolysis,2013,24(3):243-246.
• [12] Levy GA,Liu MF,Ding JW,et a1. Molecular and functional analysis of the human prothrombinase gene(HFGL2)and its role in Viral hepatitis[J]. American Journal of Pathology,2000,156(4):1217-1225.
• [13] Zheng Z,Zhang F,Gao D,et al. Gene expression profiles of rat MMECs with different glucose levels and FGL2 gene silencing[J]. Diabetes-Metabolism:ResearchandReviews,2018,34(8):e3058.
• [14] Li WZ,Yang Y,Liu K,et al. FGL2 prothrombinase contributes to the early stage of coronary microvascular obstruction through a fibrin-dependent pathway[J]. International journal of cardiology,2019,274:27-34.
• [15]刘雷，庄丽维.大黄素对急性坏死性胰腺炎的防治作用[J].中国实验诊断学，2012,5(16):852-855.
• [16]石占利，方堃，孙静，等.大黄素对重症急性胰腺炎大鼠胰腺细胞凋亡的干预作用[J].中华全科医学，2017,15(11):1830-1834.