参附注射液对肠缺血再灌注时大鼠肾组织iNOS表达的影响The Effect of Shenfu Injection on Expression of iNOS in Renal Failure Induced by Intestinal Ischemia-Reperfusion in Rats
贾一帆,任汉琳,夏中元,黄赐雄
摘要(Abstract)：
目的观察参附注射液(SFI)对大鼠肠缺血再灌注(IRI)肾组织内诱生型一氧化氮合酶(iNOS)表达的影响,初步探讨其肾保护作用机制。方法采用钳闭大鼠肠系膜上动脉复制缺血再灌注模型。健康成年雄性大鼠随机分为缺血再灌注+生理盐水组(IRL+SFI组)、SFI预处理组(IRI+NS组)和假手术组(C组)。IRI+SFI组缺血前30min恒速泵入SFI10ml/kg,阻断SMA造成肠缺血1h后再开放;IRI+NS组在缺血前30min泵持续注入等量NS。应用免疫组织化学方法和图像分析系统检测肾组织中INOS的表达和分布,比较各组血清肌酐(Cr)、尿素氮(BUN),同时观察肾脏组织形态学改变。结果与C组比较,IRI+NS组INOS表达明显增强(均P<0.01),血清Cr、BUN明显增加(P<0.01);与IRI+NS组比较,IRI+SFI组iNOS表达明显降低(均P<0.05),血清Cr、BUN明显降低。病理学检查显示SFI能明显减轻肠I/R导致的肾组织的病理损害。结论SFI明显减轻肠I/R所致的肾组织的损伤,其机制与抑制iNOS的表达有关。
关键词(KeyWords)： 肾损伤;缺血再灌注;肠;诱生型一氧化氮合酶;参附注射液
基金项目(Foundation): 湖北省教育厅立项课题(No.2003-125)
作者(Author): 贾一帆,任汉琳,夏中元,黄赐雄
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