夏炳江,侯明生,张磊,胡松峰,王宁

• 1:浙江省绍兴市中医院
• 2:浙江中医药大学

摘要(Abstract)：

目的观察五福饮对大鼠退变椎间盘聚蛋白聚糖与Ⅱ型胶原表达的影响。方法将48只SD大鼠分成空白对照组、模型组及五福饮组各16只,模型组及五福饮组大鼠应用细针纤维环穿刺结合肿瘤坏死因子-α椎间盘内注射进行造模,造模成功后3组大鼠分别给予生理盐水、生理盐水及五福饮灌胃治疗8周。采用Micro-CT检测脊柱退变情况,HE染色观察椎间盘退变特征,RT-PCR检测椎间盘聚蛋白聚糖与Ⅱ型胶原mRNA的表达。结果造模后,大鼠椎间盘出现明显退变特征,表明造模成功。灌服五福饮8周后,大鼠造模椎间隙相对高度及组织学退变均有改善。五福饮组大鼠与模型组大鼠相比较,椎间盘聚蛋白聚糖与Ⅱ型胶原mRNA表达均有明显提高(P<0.05)。结论五福饮可防治椎间盘退变,提高聚蛋白聚糖与Ⅱ型胶原表达可能是其作用机制。

关键词(KeyWords)： 椎间盘退变;五福饮;聚蛋白聚糖;Ⅱ型胶原;动物实验

Abstract:

Keywords:

基金项目(Foundation): 浙江省中医药科技计划项目(2017ZB091);; 浙江省绍兴市科技计划项目(2015B70060)

作者(Author): 夏炳江,侯明生,张磊,胡松峰,王宁

参考文献(References)：

• [1]Freemont AJ.The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain[J].Rheumatology,2009,48(1):5-10.
• [2]黄吉军,邵增务,熊蠡茗.细胞老化与椎间盘退变[J].国际骨科学杂志,2009,30(4):226-228.
• [3]莫伟,许金海,叶洁,等.腰椎间盘突出症中医治疗方法的研究进展[J].中国中医急症,2016,25(3):474-476.
• [4]王小平.论《内经》确立五脏概念的文化基础[J].中华中医药学刊,2013,31(6):1227-1229.
• [5]张红玉,杨锋,王波波,等.五福饮增强化疗药抗肿瘤作用及保护骨髓造血功能的实验研究[J].浙江中医药大学学报,2014,38(6):682-685.
• [6]夏炳江,张磊,胡松峰,等.纤维环穿刺结合TNF-α注射构建大鼠尾椎间盘退变模型的实验研究[J].中国中医急症,2017,26(7):1150-1154.
• [7]Keorochana G,Johnson JS,Taghavi CE,et al.The effect of needle size inducing degeneration in the rat caudal disc:evaluation using radiograph,magnetic resonance imaging,histology,and immunohistochemistry[J].Spine J,2010,10(11):1014-1023.
• [8]庞浩,刘祥准.五福饮对柔道运动员抗疲劳效果的研究[J].中医临床研究,2014,6(34):1-3,6.
• [9]谢席胜,刘衡川,樊均明,等.人参皂甙Rbl对转化生长因子-β1诱导的肾小管上皮细胞p47phox的表达及细胞外基质的影响[J].四川大学学报:医学版,2009,40(1):106-110.
• [10]李广波,秦娜,林瑞霞,等.地黄提取物对多柔比星肾病大鼠肾脏的保护作用机制[J].实用儿科临床杂志,2010,25(23):1815-1818.
• [11]王艳琴.当归多糖对肺纤维化大鼠肺功能、细胞外基质及病理形态学的影响[D].兰州:兰州大学,2010.
• [12]王郁金,苏衍进,郑广娟.白术挥发油对小鼠H22肝癌淋巴道转移模型的影响[J].现代中医药,2009,29(4):74-75.
• [13]宁士龙.RECK蛋白在异甘草素抑制乳腺癌细胞侵袭转移中的作用[D].南京:南京医科大学,2016.
• [14]Marchand F,Ahmed AM.Investigation of the laminate structure of lumbar disc anulus fibrosus[J].Spine,1990,15(5):402-410.
• [15]Lotz JC.Mechanobiology of the intervertebral disc[J].Biochem Soc Trans,2002,30(6):853-858.
• [16]Ahsan R.Biochemical and morphological changes in herniated human intervertebral disc[J].J Orthop Sci,2001,6(6):510-508.
• [17]Watanabe,H,Y.Yamada,and K.Kimata,Roles of aggrecan,a large chondroitin sulfate proteoglycan,in cartilage structure and function[J].J Biochem,1998,124(4):687-693.
• [18]Scott JE.Proteoglycan:collagen interactions and subfibrillar structure in collagen fibrils.Implications in the development and ageing of connective tissues[J].J Anat,1990,169:23-35.
• [19]Urban MR.Intervertebral disc composition in neuromuscular scoliosis:changes in cell density and glycosaminoglycan concentration at the curve apex[J].Spine,2001,26(6):610-617.
• [20]Roberts S.1991 Volvo Award in basic sciences.Collagen types around the cells of the intervertebral disc and cartilage end plate:an immunolocalization study[J].Spine,1991,16(9):1030-1038.
• [21]Luo W,Guo C,zheng J,et a1.Aggrecan from start to finish[J].J Bone Miner Metab,2000,18(2):51-56.
• [22]熊晓芊,邵增务,杨述华.聚集蛋白聚糖与椎间盘退变的研究进展[J].中国脊柱脊髓杂志,2005,15(1):54-57.
• [23]Bradford DS,Cooper KM,Oegema TR.Chymopapain,chemonucleolysis,and nucleus pulposus regenera tion[J].J Bone Joint Surg Am,1983,65(9):1220-1231.
• [24]Takegami K,An HS,Kumano F,et al.Osteogenic protein-1 is most effective in stimulating nucleus pulposus an annulus fibrosus cells to repair their matrix after chondroitinase ABCinduced in vitro chemonucleolysis[J].Spine J,2005,5(3):231-238.