申定珠,邢三丽,陈川,吴蓓玲,迟惠英,陈久林

• 1:上海市中医老年医学研究所

摘要(Abstract)：

目的观察首参颗粒对ApoE~(-/-)小鼠Toll样受体4(TLR4)、单核细胞趋化因子-1(MCP-1)、细胞间黏附分子-1(ICAM-1)的影响。方法采用高脂饮食喂饲ApoE~(-/-)小鼠复制动脉粥样硬化(AS)模型,并给予首参颗粒、阿托伐他汀干预15周,采用Western blotting法检测小鼠主动脉TLR4蛋白表达,ELISA法检测血清MCP-1、ICAM-1表达,结合血脂及组织病理学检测。结果与正常组比较,高脂饮食喂饲ApoE~(-/-)小鼠可致明显的AS病变,TLR4、MCP-1、ICAM-1表达上调及TC、LDL-C含量升高;经首参颗粒干预,AS斑块面积显著减小,TLR4、MCP-1、ICAM-1表达下调,TC、LDL-C含量降低(P<0.05或P<0.01)。结论首参颗粒可通过下调ApoE~(-/-)小鼠主动脉TLR4蛋白表达与血清MCP-1、ICAM-1水平进而有效干预治疗AS。

关键词(KeyWords)： 动脉粥样硬化;TLR4;MCP-1;ICAM-1;首参颗粒

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81202731,81373706);; 上海市自然科学基金项目(16ZR1433900);; 上海市卫计委科研项目(201640217)

作者(Author): 申定珠,邢三丽,陈川,吴蓓玲,迟惠英,陈久林

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