沈俊逸,方邦江,赵智明,刘春丽,壮雨雯,徐文俊,蔡辉

• 1:南京军区南京总医院
• 2:上海中医药大学附属龙华医院

摘要(Abstract)：

目的观察复元醒脑汤对microRNA-503及CCNE1、CDC25A蛋白表达的影响,探讨复元醒脑汤促进糖尿病脑梗死血管新生的分子机制。方法 150只健康雄性SD大鼠随机分为空白对照组、假手术组、模型组、西药治疗组、中药治疗组,每组30只。采用腹腔注射链脲佐菌素(STZ)后予以高能量高脂肪饲料喂养2周的方法复制糖尿病大鼠模型,制模后采用线栓法制备糖尿病脑梗死动物模型。完成造模后,中药治疗组灌胃复元醒脑汤;西药治疗组灌胃二甲双胍、尼莫地平;空白对照组、假手术组、模型组均灌胃等量的0.9%氯化钠注射液。各组连续灌胃14 d后处死取材。免疫荧光法检测脑梗死区域的血管新生、微循环观察仪检测脑梗死区域血管微循环状况、实时荧光定量PCR法检测脑组织microRNA-503、CCNE1、CDC25A基因表达水平,利用Western blotting检测CCNE1、CDC25A蛋白表达水平。结果与模型组相比,中药治疗组和西药治疗组的脑梗死区域都出现血管新生增多,血管管径增粗,血流加快,蛋白渗漏减少,少见白细胞黏附现象且中药治疗组优于西药治疗组;各实验组脑组织的CCNE1、CDC25A基因及蛋白表达水平由高到低依次为:空白对照组、假手术组、中药治疗组、西药治疗组、模型组。各实验组脑组织microRNA-503基因的表达水平由低到高依次是:空白对照组、假手术组、中药治疗组、西药治疗组、模型组,中药治疗组和西药治疗组均显著低于模型组(P <0.01)。结论复元醒脑汤通过上调CCNE1、CDC25A的表达,从而抑制microRNA-503的表达水平,达到促进梗死组织区域的血管新生,改善脑部微循环。

关键词(KeyWords)： 糖尿病脑梗死;脑部微循环;复元醒脑汤;血管新生;microRNA-503

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81273725)

作者(Author): 沈俊逸,方邦江,赵智明,刘春丽,壮雨雯,徐文俊,蔡辉

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