倪钰莹,孙彤彤,范淑月,吴璐蔚,高婧,崔颖,刘敏

• 1:北京中医药大学

摘要(Abstract)：

目的 探究麻黄细辛附子汤对Th2偏移、Treg细胞功能及Notch信号通路的干预作用。方法 体外培养CD4+T细胞，流式细胞仪鉴定纯度，使用白细胞介素-4(IL-4)、干扰素-γ抗体（Anti-IFN-γ）诱导建立Th2细胞分化模型，DAPT及麻黄细辛附子汤干预24 h后收集上清及细胞，ELISA检测白细胞介素-5(IL-5)、干扰素-γ(IFN-γ)、转化生长因子β1(TGF-β1)含量，RT-PCR检测T细胞特异性转录因子（T-bet）、转录因子结合蛋白-3(GATA-3)、叉头翼状螺旋转录因子3(FOXP3)、Notch1 mRNA表达，Western blotting检测T-bet、GATA-3、FOXP3、Notch1蛋白表达。结果 1）较之空白组，模型组IL-5含量升高，IFN-γ、TGF-β1含量降低（P <0.01）；较之模型组，各给药组IL-5含量降低，IFN-γ、TGF-β1含量升高（P <0.01）。2）较之空白组，模型组Tbet、FOXP3 mRNA表达降低，GATA-3、Notch1 m RNA表达升高（P <0.01）；较之模型组，各给药组T-bet、FOXP3 mRNA表达升高，GATA-3、Notch1 m RNA表达降低（P <0.01）。3）较之空白组，模型组T-bet、FOXP3蛋白表达降低，GATA-3、Notch1蛋白表达升高（P <0.01）；较之模型组，各给药组T-bet、FOXP3蛋白表达升高，GATA-3、Notch1蛋白表达降低（P <0.01）。结论 1）麻黄细辛附子汤能够下调IL-5分泌和GATA-3表达，促进IFN-γ分泌和T-bet表达，纠正Th2偏移。2）麻黄细辛附子汤有助于Treg细胞分化增殖，能够促进TGF-β1分泌和FOXP3表达。3）麻黄细辛附子汤能够通过抑制Notch1表达，促进Treg细胞功能发挥，纠正Th2偏移。

关键词(KeyWords)： 变应性鼻炎;麻黄细辛附子汤;Notch信号通路;Treg细胞;Th2偏移

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（81774375）

作者(Author): 倪钰莹,孙彤彤,范淑月,吴璐蔚,高婧,崔颖,刘敏

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