苦参碱对人胃癌细胞NCI-N87体外的增殖抑制作用The Inhibitory Effect of Matrine on the Proliferation of Human Gastric Cancer Cell Line NCI-N87 in Vitro
王中林,蒋平,于永洋
摘要(Abstract)：
目的探讨不同浓度苦参碱对体外培养的人胃癌细胞NCI-N87的增殖抑制作用。方法NCI-N87细胞经不同浓度苦参碱处理后,采用CCK-8法检测药物对肿瘤细胞的抑制作用,观察细胞形态学改变,检测细胞DNA分布。结果苦参碱浓度>3.0mg/ml,细胞毒作用较大;浓度在0.5～2.0mg/ml时,对肿瘤细胞的生长有抑制作用,且呈时间剂量依赖性;浓度在0.1mg/ml时,对肿瘤细胞生长几乎无抑制作用。苦参碱作用后可增加G1期细胞所占的百分比,阻止细胞进入S期。结论苦参碱对人胃癌细胞NCI-N87增殖有抑制作用,并能阻抑细胞周期的进程。
关键词(KeyWords)： 苦参碱;胃癌;细胞增殖
基金项目(Foundation): 3浙江省温州市科技局资助项目(No.S990323-3);; 浙江省卫生厅资助项目(No.2000A037)
作者(Author): 王中林,蒋平,于永洋
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