胡爽,何泽云,徐文峰,唐群,董翔

• 1:湖南中医药大学
• 2:湖南中医药大学第一附属医院

摘要(Abstract)：

目的探讨六味地黄汤对慢性肾衰竭大鼠肾脏Bax和Bcl-2表达的影响。方法将45只雄性SD大鼠随机分为空白组(10只)、假手术组(10只)及手术组(25只),手术组予以5/6肾切除造模,造模成功后随机分为模型组及治疗组。治疗组予六味地黄汤灌胃,其余3组予等体积蒸馏水灌胃。干预8周后检测各组大鼠血肌酐、血尿素氮、尿蛋白定量水平;HE、Masson染色观察大鼠肾脏组织形态学变化;免疫组化及实时荧光定量PCR分别检测大鼠Bax及Bcl-2蛋白及mRNA的表达水平。结果空白组及假手术组大鼠肾脏未观察到明显病理变化,模型组存在不同程度的肾小管及肾间质病变,治疗组较模型组病灶明显减轻;模型组血肌酐、血尿素氮、尿蛋白定量水平明显升高(P <0.01),治疗组较模型组降低(P <0.05);Bax和Bcl-2主要在肾小管区表达,模型组Bax表达水平明显升高(P <0.01),Bcl-2表达水平明显降低(P <0.01);与模型组比较,治疗组Bax表达水平降低(P <0.05),Bcl-2表达水平升高(P <0.05)。结论六味地黄汤可通过调控凋亡相关蛋白Bax和Bcl-2的表达,改善慢性肾衰竭大鼠肾纤维化程度,延缓肾功能进展。

关键词(KeyWords)： 慢性肾衰竭;六味地黄汤;细胞凋亡;Bax;Bcl-2;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（81503442）;; 湖南中医药大学研究生创新课题项目（2019CX13）

作者(Author): 胡爽,何泽云,徐文峰,唐群,董翔

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