彭洪卫,林如辉,邵丽,刘婧

• 1:江苏省徐州市第一人民医院
• 2:福建中医药大学科技转化与创新中心

摘要(Abstract)：

目的 观察电针百会、神庭穴对局灶脑缺血再灌注（MCAO）大鼠海马CA1区核转录因子κB(NF-κB p56的表达变化，探讨其对MCAO大鼠学习记忆功能的改善作用。方法 采用线栓法建立MCAO大鼠模型。50只成年雄性SD大鼠按照随机数字表法分为假手术组（n=14）、模型组（n=12）、电针组（n=12）和非穴组（n=12）。电针组大鼠选取百会、神庭穴进行电针刺激，非穴组大鼠选取双胁下非经非穴进行电针刺激，共干预7 d。磁共振扫描计算大鼠脑梗死体积；跳台实验评估大鼠学习记忆能力；免疫组化技术检测大鼠海马CA1区NF-κB p65的表达情况，Western blotting分析脑组织中NF-κB p65、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的水平。结果 核磁共振扫描显示电针组大鼠脑梗死体积较模型组、非穴组明显减小（P <0.05）。干预7 d后，电针组大鼠跳台实验潜伏期较模型组和非穴组明显增加（P <0.05）。免疫组化结果示，模型组和非穴组大鼠海马CA1区细胞质和细胞核表达均显著高于假手术组，且模型组与非穴组之间无统计学差异（P> 0.05）。干预7 d后，电针组细胞核中NF-κB p65的胞核、胞质表达水平均较模型组下降（P <0.05）。Western blotting结果表明电针组大鼠脑组织中NF-κB p65、IL-1β、IL-6含量均明显低于模型组和非穴组（P <0.05）。结论 电针治疗能够抑制大鼠局灶脑缺血再灌注后海马CA1区NF-κB p65及炎症因子IL-1β、IL-6的表达，从而改善大鼠的学习记忆功能。

关键词(KeyWords)： 脑缺血再灌注;电针;学习记忆;核转录因子-κB;大鼠

Abstract:

Keywords:

基金项目(Foundation): 江苏省老年健康科研项目（LKM2022045）;; 徐州市卫生健康委科技项目（XWKYHT20220084）

作者(Author): 彭洪卫,林如辉,邵丽,刘婧

参考文献(References)：

• [1] MAIDA CD,NORRITO RL,DAIDONE M,et al. Neuroinflammatory mechanisms in ischemic stroke:Focus on cardioembolic stroke,background,and therapeutic approaches[J]. Int J Mol Sci,2020,21(18):6454.
• [2] WANG B,MAO L,SHEN T,et al. Effects of combined intervention of physical exercise and cognitive training on cognitive function in stroke survivors with vascular cognitive impairment:a randomized controlled trial[J]. Clin Rehabil,2019,33(1):54-63.
• [3] DU YZ,ZHANG LL,LIU W,et al. Effect of acupuncture treatment on post-stroke cognitive impairment:A randomized controlled trial[J]. Medicine,2020,99(51):e23803.
• [4]曲艳吉，卓琳，詹思延.中国脑卒中后认知障碍流行病学特征的系统评价[J].中华老年心脑血管病杂志，2013,15(12):1294-1301.
• [5] LIU HX,WU X,LUO JN,et al. Pterostilbene attenuates astrocytic inflammation and neuronal oxidative injury after ischemia-reperfusion by inhibiting NF-κB phosphorylation[J].Front Immunol,2019,10:2408.
• [6] WANG HL,LIU FL,LI RQ,et al. Electroacupuncture improves learning and memory functions in a rat cerebral ischemia/reperfusion injury model through PI3K/Akt signaling pathway activation[J]. Neural Regen Res,2021,16(6):1011-1016.
• [7] WEN T,ZHANG XF,LIANG SX,et al. Electroacupuncture ameliorates cognitive impairment and spontaneous low-frequency brain activity in rats with ischemic stroke[J]. J Stroke Cerebrovasc Dis,2018,27(10):2596-2605.
• [8]余曙光，徐斌.实验针灸学[M]. 2版.北京：人民卫生出版社，2016.
• [9] KWON CY,LEE B,KIM SH. Efficacy and underlying mechanism of acupuncture in the treatment of posttraumatic stress disorder:A systematic review of animal studies[J]. J Clin Med,2021,10(8):1575.
• [10] ZHAN J,PAN RH,GUO YH,et al. Acupuncture at Baihui(GV 20)and Shenting(GV 24)combined with basic treatment and regular rehabilitation for post-stroke cognitive impairment:a randomized controlled trial[J]. Chinese Acupuncture and Moxibustion,2016,36(8):803-806.
• [11] LIN RH,LIN YK,TAO J,et al. Electroacupuncture ameliorates learning and memory in rats with cerebral ischemia-reperfusion injury by inhibiting oxidative stress and promoting p-CREB expression in the hippocampus[J]. Mol Med Rep,2015,12(5):6807-6814.
• [12] ARROYO GARCIA LE,TENDILLA BEELTáN H,Vázquez Roque RA,et al. Amphetamine sensitization alters hippocampal neuronal morphology and memory and learning behaviors[J]. Mol Psychiatry,2020,26(9):4784-4794.
• [13] MCDONALD AJ,MOTT DD. Functional neuroanatomy of amygdalohippocampal interconnections and their role in learning and memory[J]. J Neurosci Res,2017,95(3):797-820.
• [14] SILVA B,SOUSA L,MIRANDA A,et al. Memory deficit associated with increased brain proinflammatory cytokine levels and neurodegeneration in acute ischemic stroke[J]. Arq Neuropsiquiatr,2015,73(8):655-659.
• [15] WAN YJ,XU J,MA DQ,et al. Postoperative impairment of cognitive function in rats:a possible role for cytokine-mediated inflammation in the hippocampus[J]. Anesthesiology,2007,106(3):436-443.
• [16] KOHMAN RA,RHODES JS. Neurogenesis,inflammation and behavior[J]. Brain Behav Immun,2013,27(1):22-32.
• [17] RIDDER DA,SCHWANINGER M. NF-κB signaling in cerebral ischemia[J]. Neuroscience,2009,158:995-1006.
• [18] HWANG CJ,YUN HM,JUNG YY,et al. Reducing effect of IL-32α in the development of stroke through blocking of NF-κB,but enhancement of STAT3 Pathways[J]. Mol Neurobiol,2015,51(2):648-660.
• [19] PHUAGKHAOPONG S,OSPONDPANT D,KASEMSUK T,et al. Cadmium-induced IL-6 and IL-8 expression and release from astrocytes are mediated by MAPK and NF-κB pathways[J]. Neurotoxicology,2017,60:82-91.