黄坡,胡晓,方晓磊,郭玉红,刘清泉

• 1:北京中医药大学东方医院
• 2:首都医科大学附属北京中医医院
• 3:北京市中医药研究所

摘要(Abstract)：

目的 借助网络药理学分析技术研究强心一号复方干预脓毒症心功能障碍的作用机制。方法 首先通过检索TCMSP数据库获取黄芪、茯苓、丹参、水红花子和五味子的主要活性成分及其靶点；检索GeneCards、OMIM、TTD、DrugBank 4个在线数据库来获取脓毒症心功能障碍的潜在靶点；获取强心一号复方和脓毒症心功能障碍的交集靶点并上传至在线网站中制作Venn图；使用String在线网站绘制蛋白互作网络图，然后在Cytoscape3.10.0软件中进行可视化分析；借助Cytoscape3.10.0软件构建“强心一号复方-Components-TargetsSIMD-Pathways”网络图；使用DAVID软件进行GO和KEGG分析。结果 共获得药物靶点244个，药物靶点与疾病靶点交集后获得114个靶点，核心靶点有Akt1、MMP3、VEGFA和STAT3等。GO分析显示，强心一号复方参与的生物过程主要集中在炎性反应、氧化应激和细胞凋亡方面，参与的细胞组分主要有线粒体和质膜等，而参与的分子功能主要是蛋白激酶活性等；KEGG富集分析显示，强心一号复方干预SIMD的信号通路主要富集在癌症相关信号转导通路、PI3K/Akt、人巨细胞病毒感染、MAPK、TNF和凋亡等信号通路。结论 PI3K/Akt、MAPK和凋亡信号通路可能是强心一号复方调控脓毒症心功能障碍的关键信号通路。

关键词(KeyWords)： 脓毒症心功能障碍;强心一号复方;网络药理学;作用机制

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（82205070,81973608）;; 睿E（睿意）急诊医学科研专项基金资助项目（2021-睿E-010）;; 北京中医药大学2021年度新教师启动基金项目（2021-YJB-XJSJJ-065）

作者(Author): 黄坡,胡晓,方晓磊,郭玉红,刘清泉

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