朱满刚,伍万,杨月,叶远玲,彭伟献

• 1:浙江省杭州市中医院
• 2:浙江省杭州市临平区中西医结合医院

摘要(Abstract)：

目的 观察参附注射液对脓毒症大鼠肠黏膜屏障功能的影响。方法 将40只大鼠随机分为假手术组、脓毒症组、参附注射液低剂量组和高剂量组，每组各10只。行盲肠结扎穿孔术制备重度脓毒症模型，假手术组仅行开腹手术后即予关闭腹腔。术后10 min内经尾静脉推注药物：模型组给予生理盐水10 mL/kg；参附低剂量组给予5 mL/kg参附注射液+生理盐水5 m L/kg稀释液；参附高剂量组给予10 mL/kg参附注射液。8 h后处死大鼠，以ELISA检测各组大鼠血清肿瘤坏死因子-ɑ（TNF-ɑ）及白细胞介素-10(IL-10)浓度、回肠组织丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性，HE染色观察回肠组织病理改变（Chiu′s评分），TUNEL法检测回肠黏膜上皮细胞凋亡率（AI），免疫组化检测促凋亡蛋白Bax与抗凋亡蛋白Bcl-2,Western blotting检测跨膜紧密连接蛋白occludin水平。结果 与假手术组比较，模型组INF-α、MDA、Chiu′s评分、AI及Bax均显著增高，IL-10、SOD、Bcl-2、ooccludin均显著降低，差异均有统计学意义（P <0.05）；与模型组比较，参附低剂量组、参附高剂量组TNF-α、MDA、Chiu′s评分、AI均显著降低，IL-10、SOD显著增高，差异均有统计学意义（P <0.05）；与参附低剂量组比较，参附高剂量组TNF-α、MDA、Chiu′s评分、AI、Bax均显著降低，IL-10、SOD、Bcl-2、occludin均显著增高，差异均有统计学意义（P <0.05）。结论 参附注射液可显著改善重度脓毒症模型大鼠的肠黏膜屏障功能，其作用与参附注射液的剂量成正相关。

关键词(KeyWords)： 脓毒症;肠黏膜屏障;参附注射液;大鼠

Abstract:

Keywords:

基金项目(Foundation): 浙江省基础公益研究计划项目（LGF19H270006）

作者(Author): 朱满刚,伍万,杨月,叶远玲,彭伟献

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