高江宝,郝晓东,李超云,宋斐,吴少丽,杨英艺

• 1:广东省广州市番禺区中医院

摘要(Abstract)：

目的 探讨扶正解毒方减轻脓毒血症肠损伤的作用及机制。方法 将60只大鼠随机分为假手术组、模型组、Rho激酶抑制剂组（Y-27632）、扶正解毒方低剂量组、扶正解毒方高剂量组、蒙脱石散组，每组各10只。药物干预4周后，收集大鼠血清及结肠组织，HE染色观察各组大鼠结肠组织形态学改变，ELISA法比较各组大鼠血清炎症因子水平、氧化应激水平，Western blottng法检测各组大鼠结肠组织Rho/Rock信号通路相关蛋白水平。结果 低剂量、高剂量扶正解毒方均可以明显改善大鼠脓毒血症肠损伤，有效降低大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、HMBG1水平（P <0.05），并且血清及结肠组织丙二醛（MDA）、超氧化物歧化酶（SOD）的含量也明显下降（P <0.05），扶正解毒方低剂量、高剂量组大鼠结肠组织Rho、Rock蛋白的表达显著下调（P <0.05）,ZO-1、Occludin蛋白的表达显著上调（P <0.05）。结论 扶正解毒方可以通过抑制Rho/Rock信号通路，减少肠道炎症和氧化应激，从而改善大鼠脓毒血症引起的肠道损伤，其作用呈一定的剂量依赖性。

关键词(KeyWords)： 脓毒血症;肠损伤;扶正解毒方;Rho/Rock信号通路;大鼠

Abstract:

Keywords:

基金项目(Foundation): 广东省中医药局科研项目（20212184）

作者(Author): 高江宝,郝晓东,李超云,宋斐,吴少丽,杨英艺

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