蔡婷,潘力弢,李蒋凤,谭亮,张梦,李晶晶,樊光辉

• 1:湖北中医药大学
• 2:广东省深圳市第二人民医院
• 3:深圳大学附属华南医院

摘要(Abstract)：

目的 观察三七皂苷R1对血管平滑肌细胞（VSMC）迁移及p38 MAPK蛋白的影响。方法 用AngⅡ诱导VSMC迁移建立细胞迁移模型，将迁移的VSMC随机分为对照组和三七皂苷R1低、中、高剂量组（5、10、20μmol/L）。用细胞划痕实验检测VSMC迁移面积，Western blotting法检测MMP2、MMP9、磷酸化p38 MAPK及p38 MAPK蛋白表达。结果 与对照组相比，三七皂苷R1中、高剂量组均能明显减少VSMC的细胞迁移面积及MMP2、MMP9迁移蛋白表达（P <0.05），且呈剂量依赖性；与对照组相比，三七皂苷R1中、高剂量组均能明显减少p38 MAPK蛋白磷酸化（P <0.05），且呈剂量依赖性。结论 三七皂苷R1抑制了AngⅡ诱导的VSMC迁移面积，同时下调MMP2和MMP9迁移蛋白，且呈剂量依赖性，其机制可能与降低p38 MAPK蛋白磷酸化有关。

关键词(KeyWords)： 血管平滑肌细胞;迁移;三七皂苷R1;p38 MAPK;MMP2;MMP9

Abstract:

Keywords:

基金项目(Foundation): 深圳市高等院校稳定支持计划（20200831114632002）

作者(Author): 蔡婷,潘力弢,李蒋凤,谭亮,张梦,李晶晶,樊光辉

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