赵新军,李荣,吴伟,卿立金,罗川晋,褚庆民,李靖,都治伊,邱艺俊

• 1:广州中医药大学第一附属医院

摘要(Abstract)：

目的观察清热活血方对心梗后心室重构的改善作用并对其可能机制进行探讨。方法将心梗造模成功的50只Wistar大鼠随机分成5组,另设假手术组,共6组:假手术组、心梗模型组、阳性对照组及清热活血方高剂量组、中剂量组和低剂量组,分别给予相应干预,8周后处死大鼠,评估清热活血方对大鼠生存率的影响,通过心脏结构及心功能判断清热活血方对心室重构的改善情况,通过Western blotting法检测心肌组织中多胺相关蛋白鸟氨酸脱羧酶(ODC)、精脒/精胺乙酰基转移酶(SSAT)的表达,通过高效液相色谱法检测心肌组织中多胺的含量。结果清热活血方可明显提高心梗大鼠的生存率,和模型组对比差异有统计学意义;心脏彩超结果显示,心梗模型组心功能明显下降,接近假手术组2倍,培哚普利组、清热活血方高、中剂量组心功能明显提高。Western blotting半定量分析显示,模型组中ODC和SSAT蛋白表达均有明显增强,同模型组对比,培哚普利组和清热活血方高、中剂量组能降低ODC的表达,同时也增强了SSAT蛋白表达;高效液相色谱法检测发现,心梗模型组的左室非梗死区心肌细胞内多胺各成分含量水平明显升高,总多胺池水平也明显增高(P<0.05),清热活血方组腐胺、精脒及精胺的含量显著降低,有浓度依赖趋势。结论 1)清热活血方是改善心梗后心室重构的有效药物。2)清热活血方抑制心室重构的机制与调节多胺和多胺相关蛋白通路有关。

关键词(KeyWords)： 心梗后心室重构;清热活血方;多胺系统;鸟酸脱羧酶;精脒/精胺乙酰基转移酶;大鼠

Abstract:

Keywords:

基金项目(Foundation): 广东省中医药局科研项目(20171086)

作者(Author): 赵新军,李荣,吴伟,卿立金,罗川晋,褚庆民,李靖,都治伊,邱艺俊

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