孙中美,丁庞华,王文婷,史瑞,裴文静,郭一,谢添弘,姜慧,石磊,原文静,毛堂友,李军祥

• 1:北京中医药大学东方医院
• 2:北京中医药大学
• 3:北京市羊坊店医院
• 4:北京中医药大学东直门医院

摘要(Abstract)：

目的研究清肠温中方调控DSS诱导溃疡性结肠炎(UC)大鼠Th17/Treg免疫平衡及肠黏膜屏障的作用机制。方法 24只SPF级雄性SD大鼠,按体质量依照随机数字表将其随机分为空白组、模型组、清肠温中方组。空白组自由饮用去离子水,同时予去离子水灌胃;模型组和清肠温中方组自由饮用4.5%DSS溶液制备UC模型,同时模型组给予去离子水,清肠温中方组予清肠温中方灌胃。7 d后麻醉大鼠后取血及结肠组织,应用Real time-PCR法检测结肠miR-675-5p、VDR m RNA、RORγt mRNA、Foxp3 m RNA的表达,ELISA法检测血清IL-10、IL-17的表达,免疫组织化学法检测ZO-1、Occludin的蛋白表达及分布。结果与空白组比较,DSS诱导的UC大鼠结肠mi R-675-5p、RORγt mRNA及血清IL-17的表达较空白组明显升高(P <0.05或P <0.01),VDR mRNA、Foxp3 mRNA、ZO-1、Occludin、血清IL-10的表达较空白组明显降低(P <0.05或P <0.01);清肠温中方干预后,大鼠结肠mi R-675-5p、RORγt mRNA、血清IL-17的表达较模型组明显降低(P <0.05),VDR mRNA、Foxp3 mRNA、ZO-1、Occludin、血清IL-10的表达较模型组明显升高(P <0.05或P <0.01)。结论清肠温中方可能通过降低mi R-675-5p的表达,靶向调控VDR信号通路,从而调控溃疡性结肠炎Th17/Treg免疫平衡、修复肠黏膜屏障损伤,达到治疗UC的目的。

关键词(KeyWords)： 溃疡性结肠炎;清肠温中方;miR-675-5p/VDR信号通路;肠黏膜屏障;Th17/Treg免疫平衡

Abstract:

Keywords:

基金项目(Foundation): 2018年北京中医药大学自主课题青年教师项目(2018-JYBZZ-JS097)

作者(Author): 孙中美,丁庞华,王文婷,史瑞,裴文静,郭一,谢添弘,姜慧,石磊,原文静,毛堂友,李军祥

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