电针对脑缺血再灌注大鼠缺血区皮质TNF-α mRNA表达及TNF-α含量影响The Effects of Electroacupuncture on the Expression of TNF-α mRNA and the Level of TNF-α in Ischemic Penumbra in Cortices of Rats with Cerebral Ischemia-Reperfusion
丁德光,李家康,罗惠平,周仲愉,焦扬,韦丹
摘要(Abstract)：
目的通过观察电针对脑缺血再灌注大鼠缺血区皮质TNF-α mRNA的表达和TNF-α含量的影响以探讨电针对缺血性脑损伤的保护机制。方法采用大脑中动脉线栓法制作模型;电针大鼠的水沟、内关,运用逆转录-多聚酶链反应(RT-PCR)法检测缺血区皮质TNF-α mRNA表达和放射免疫法检测皮质TNF-α含量。结果针刺各组缺血皮质中TNF-α mRNA表达和TNF-α含量与模型组相比均降低,有统计学意义。结论电针通过抑制TNF-α mRNA的表达减少TNF-α合成,以对抗缺血再灌注脑组织神经损伤。
关键词(KeyWords)： 脑缺血;再灌注损伤;电针;肿瘤坏死因子-α;大鼠
基金项目(Foundation): 湖北省教育厅科研基金资助项目(No.2004D006)
作者(Author): 丁德光,李家康,罗惠平,周仲愉,焦扬,韦丹
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