夏炳江,张磊,胡松峰,王宁,侯明生

• 1:浙江省绍兴市中医院
• 2:浙江中医药大学

摘要(Abstract)：

目的观察X线透视下纤维环穿刺结合肿瘤坏死因子-α(TNF-α)椎间盘内注射构建大鼠尾椎间盘退变模型的可行性。方法取3月龄清洁级SD大鼠96只,随机分为空白对照组(A组)、对照组(B组)和实验组(C组)各32只。C组大鼠在X线透视辅助下,利用0.6 mm穿刺针穿刺尾椎5~6椎间隙(Co5/6),穿刺成功后,以微量注射器向椎间盘内注入TNF-α(10 ng/μL)25μL。B组大鼠在椎间盘穿刺成功后注入25μL生理盐水,A组大鼠不做任何处理。采用50%机械性撤足阈值、椎间隙相对高度、椎间盘组织学改变及椎间盘聚蛋白聚糖和Ⅱ型胶原基因表达评价大鼠尾椎间盘退变情况。结果造模后,C组大鼠50%机械性撤足阈值明显降低,椎间隙相对高度逐渐下降,HE染色显示椎间盘出现明显退变表现,椎间盘蛋白聚糖及Ⅱ型胶原mRNA表达下降,表明模型构建成功,与B组大鼠相比,C组大鼠诱发的椎间盘退变过程出现更早,进展更快,程度更重。结论 X线透视下纤维环穿刺结合TNF-α椎间盘内注射是一种可行的建立大鼠尾椎间盘退变模型的方法,其所诱发的椎间盘退变过程出现更早,进展更快,程度更重。

关键词(KeyWords)： 椎间盘退变;TNF-α;动物模型;针刺

Abstract:

Keywords:

基金项目(Foundation): 浙江省绍兴市科技计划项目(2015B70060);; 浙江省中医药科技计划项目(2016ZB132)

作者(Author): 夏炳江,张磊,胡松峰,王宁,侯明生

参考文献(References)：

• [1]Chen S,Huang Y,Zhou ZJ,et al.Up-regulation of tumor necrosis factor-αand ADAMTS-5 but not ADAMTS-4 in human intervertebral cartilage endplate with modic changes[J].Spine(Phila Pa 1976),2014,39(14):E817-825.
• [2]You C,Zhu K,Liu X,et al.Tumor necrosis factor-alphadependent infiltration of macrophages into the dorsal root ganglion in a rat disc herniation model[J].Spine(Phila Pa1976),2013,38(23):2003-2007.
• [3]姜世峰,申才良.腰椎Modic改变中炎性细胞因子的效应[J].中国组织工程研究,2012,16(17):3213-3217.
• [4]Chaplan SR,Bach FW,Pogrel JW,et al.Quantitative assessment of tactile allodynia in the rat paw[J].J Neurosci Methods,1994,53(1):55-63.
• [5]Keorochana G,Johnson JS,Taghavi CE,et al.The effect of needle size inducing degeneration in the rat caudal disc:evaluation using radiograph,magnetic resonance imaging,histology,and immunohistochemistry[J].Spine J,2010,10(11):1014-1023.
• [6]Sobajima S,Kompel JF,Kim JS,et al.A slowly progressive and reproducible animal model of intervertebral disc degeneration characterized by MRI,X-ray,and histology[J].Spine(Phila Pa 1976),2005,30(1):15-24.
• [7]Liang QQ,Cui XJ,Xi ZJ,et al.Prolonged upright posture induces degenerative changes in intervertebral discs of rat cervical spine[J].Spine,2011,36(1):E14-E19.
• [8]Masuda K.Biological repair of the degenerated intervertebral disc by the injection of growth factors[J].Eur Spine J,2008,17(Suppl 4):441-451.
• [9]Rufai A,Ralphs JR,Benjamin M.The development of fibrocartilage in the rat intervertebral disc[J].Anat Embryol,1995,192(1):53-62.
• [10]Li D,Yang H,Huang Y,et al.Lumbar intervertebral disc puncture under C-arm fluoroscopy:a new rat model of lumbar intervertebral disc degeneration[J].Exp Anim,2014,63(2):227-234.
• [11]廖家新,王宸,吴小涛,等.退变腰椎终板MRI表现与其TNF-α的表达及临床疗效的相关性研究[J].现代医学,2008,36(3):201-203.
• [12]Weiler C,Nerlich AG,Bachmeier BE,et al.Ex pression and distribution of tumor necrosis factor alpha in human lumbar intervertebral discs:a study in surgical specimen and autopsy controls[J].Spine(Phila Pa 1976),2005,30(1):44-53.
• [13]Brayda-Bruno M,Tibiletti M,Ito K,et al.Advances in the diagnosis of degenerated lumbar discs and their possible clinical application[J].Eur Spine J,2014(3):S315-323.
• [14]Issy AC,Castania V,Castania M,et al.Experimental model of intervertebral disc degeneration by needle puncture in Wistar rats[J].Braz J Med Biol Res,2013,46(3):235-244.
• [15]Daly C,Ghosh P,Jenkin G,et al.A review of animal models of intervertebral disc degeneration:pathophysiology,regeneration,and translation to the clinic[J].Biomed Res Int,2016(2016):5952165.
• [16]Wang F,Jiang JM,Deng CH,et al.Expression of Fas receptor and apoptosis in vertebral endplates with degenerative disc diseases categorized as Modic type I orⅡ[J].Injury,2011,42(8):790-795.
• [17]Nagase H,Kashiwagi M.Aggrecanases and cartilage matrix degradation[J].Arthritis Res Ther,2003,5(2):94-103.