王思佳;芦殿荣;朱世杰;王宁军;

• 1:中国中医科学院望京医院

摘要(Abstract)：

目的 运用网络药理学方法探寻“半夏-黄连”药对防治化疗相关性腹泻（CID）的作用机制。方法 利用中药系统药理学数据库与分析平台（TCMSP,https：//tcmspw.com/tcmsp.php）、中医药百科全书数据库（ETCM,http：//www.tcmip.cn/ETCM/）检索药物主要有效成分并筛选药物相关靶点，通过GeneCards数据库（https：//www.genecards.org/）收集CID相关疾病基因，并将药物靶点与疾病基因进行韦恩分析得出交集基因靶点，在DAVID数据库（https：//david.ncifcrf.gov/）和KEGGMapper数据库（https：//www.genome.jp/kegg/mapper/color.html）进行富集分析，通过Cytoscape软件构建“中药-有效成分-靶点-通路”的多层次网络，筛选出核心基因。结果 半夏-黄连药对主要有效成分39个，包含269个靶点。CID基因靶点有603个，其中117个是半夏-黄连药对治疗CID的潜在靶点。通过GO功能分析和KEGG通路富集分析发现半夏-黄连药对治疗CID是通过IL-17、MAPK、TNF及PI3K/Akt信号通路发挥抗炎和抗肿瘤作用。半夏-黄连药对治疗CID的关键基因靶点为TP53、Akt1、JUN、TNF、HSP90AA1、IL-6、EGFR等。结论 本研究通过网络药理学方法发现“半夏-黄连”药对防治CID具有多靶点、多通路的治疗机制，为临床使用中医辛开苦降法治疗CID提供了理论基础。

关键词(KeyWords)： 化疗相关性腹泻;半夏-黄连;网络药理学;辛开苦降法;靶点预测

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目（81973640）

作者(Authors): 王思佳;芦殿荣;朱世杰;王宁军;

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