陈春雯,严波,单乐天,王春雷,周莉

• 1:浙江中医药大学
• 2:浙江大学医学院附属杭州市第一人民医院
• 3:浙江省肿瘤医院

摘要(Abstract)：

目的观察女贞子提取物对膝骨关节炎(KOA)大鼠关节疼痛的影响,研究其对KOA大鼠软骨的保护作用。方法 32只SD大鼠随机分为正常组、模型组、女贞子灌胃组和女贞子注射组。模型组、女贞子灌胃组和女贞子注射组均采用碘乙酸关节腔注射造模法构建大鼠KOA模型,女贞子灌胃组大鼠进行女贞子提取物灌胃处理,女贞子注射组大鼠予以关节腔内注射女贞子提取物。治疗持续4周,于最终给药后1周测定行为学数据;取各组大鼠膝关节软骨组织,进行番红O染色和Mankin′s评分,免疫组化检测Col2的表达。取大鼠原代软骨细胞,以不同浓度的女贞子提取物进行干预,采用MTT法测定女贞子提取物对软骨细胞增殖的影响,选取最佳浓度。RT-PCR测定女贞子提取物对软骨细胞中OA相关基因表达的调节作用,并进行统计分析。结果疼痛模型数据显示,模型组的压痛阈值与正常组相比显著下降(P <0.01);女贞子注射组与模型组比较有显著提高(P <0.01);女贞子灌胃组与模型组比较有提高(P> 0.05)。组织病理学结果显示,模型组的Mankin′s评分高于空白组(P <0.01);女贞子注射组Mankin′s评分较模型组显著下降(P <0.01);女贞子灌胃组与模型组比较有下降(P> 0.05)。免疫组化分析显示女贞子注射组和女贞子灌胃组软骨中Col2表达增加。女贞子提取物可促进大鼠原代软骨细胞的增殖,RT-PCR分析结果显示与TNF-α处理组相比,女贞子组显著下调Col10、MMP13的表达,上调Col2和Aggrecan的表达。结论女贞子提取物关节腔注射对软骨细胞的保护效果优于灌胃给药;女贞子提取物对大鼠KOA疼痛模型有干预作用,其机制与抑制软骨细胞肥大和分解代谢有关。

关键词(KeyWords)： 骨关节炎;软骨;女贞子;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81774331);; 浙江省中医药科技计划-青年人才基金项目(2016ZQ010)

作者(Author): 陈春雯,严波,单乐天,王春雷,周莉

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