石亚其;雷玉华;王勇;张倩;

• 1:湖北省恩施土家族苗族自治州中心医院

摘要(Abstract)：

目的 观察胡椒碱对急性心肌梗死（AMI）大鼠高迁移率族蛋白B1(HMGB1)、白细胞介素-1β(IL-1β和白细胞介素-6(IL-6)水平的影响。方法 60只SD大鼠随机分为假手术组、手术组、胡椒碱低剂量组（10 mg/kg）、胡椒碱中剂量组（20 mg/kg）及胡椒碱高剂量组（30 mg/kg）5组。建立大鼠AMI模型后，即刻给予相应剂量的胡椒碱或生理盐水灌胃，每天1次，连续灌胃7 d。TTC染色法检测心肌梗死面积，同时取各组大鼠心尖组织，病理形态学分析心肌损伤情况，ELISA法测定HMGB1、IL-1β和IL-6的含量，最后采用分子对接方法分析胡椒碱和HMGB1之间的相互作用。结果 与假手术组比较，手术组可见较大面积的心肌梗死，心肌组织可见细胞变性、细胞坏死等病理改变，HMGB1、IL-1β和IL-6水平显著升高（均P <0.05）。与手术组比较，胡椒碱低、中、高剂量组可减轻心肌梗死面积，改善心肌细胞变性坏死，降低HMGB1、IL-1β和IL-6水平，且以上改变呈剂量依赖性（均P <0.05）。分子对接分析发现胡椒碱可靶向结合HMGB1，结合能为-8.01 kcal/mol。结论 胡椒碱对急性心肌梗死心肌具有保护作用，其作用可能是通过靶向结合HMGB1，降低HMGB1、IL-1β和IL-6水平来实现。

关键词(KeyWords)： 急性心肌梗死;胡椒碱;炎性因子;IL-1β;IL-6;HMGB1;大鼠

Abstract:

Keywords:

基金项目(Foundation): 恩施州科技计划研究与开发项目（E20180003）

作者(Authors): 石亚其;雷玉华;王勇;张倩;

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