余婷,韩亚非

• 1:河北省邯郸市中医院
• 2:河北省邯郸市中心医院

摘要(Abstract)：

目的研究苦参素对大鼠脑缺血后脑线粒体的保护作用。方法采用线栓法制备局灶性脑缺血再灌注大鼠模型,设假手术组、模型组和苦参素低(25mg/kg)、中(50mg/kg)、高(100mg/kg)剂量组,各20只,分别于术前10min腹腔注射给药。24h后,测定缺血侧脑线粒体肿胀程度、膜流动性、呼吸功能,电子显微镜观察线粒体超微结构变化,测定线粒体内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,MDA含量,Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase活性,游离Ca~(2+)浓度。结果与模型组比较,经苦参素中、高剂量预处理能够明显改善大鼠脑缺血后线粒体肿胀,提高膜流动性,改善线粒体呼吸功能,抑制线粒体超微结构损伤,显著提高SOD、GSH-Px活性并降低MDA含量(P<0.05或P<0.01),显著提高Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase活性(P<0.05或P<0.01),降低游离Ca~(2+)浓度(P<0.05)。结论苦参素对脑缺血后脑线粒体损伤具有一定的保护作用,其机制可能与降低Ca~(2+)浓度、抑制氧化应激损伤以及改善ATPase活性有关。

关键词(KeyWords)： 苦参素;脑缺血;线粒体;氧化应激;ATPase;Ca2+

Abstract:

Keywords:

基金项目(Foundation): 河北省邯郸市科学技术研究与发展计划项目(1623208070ZC)

作者(Author): 余婷,韩亚非

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