朱清,姚蓓蓓,吴明华

• 1:南京中医药大学附属医院

摘要(Abstract)：

目的观察蒲参胶囊对大鼠脑缺血/再灌注后血清bFGF和BDNF蛋白表达的影响,探讨蒲参胶囊促进脑缺血后神经损伤恢复的可能作用机制。方法将健康SD大鼠随机分成蒲参胶囊低、中、高剂量组,假手术和模型组。通过线栓法建立大鼠大脑中动脉阻塞(MCAO)脑缺血再灌注模型,术后高剂量组(250 mg/200 g)、中剂量组(125 mg/200 g)、低剂量组(62.5 mg/200 g)每日予灌胃给药1次,假手术、模型组给予生理盐水灌胃。术后第1天、7天、14天分别对各组大鼠进行腹主动脉采血,并分离血清,用酶联免疫吸附剂(ELISA)测定血清中bFGF和BDNF的水平。结果与模型组相比,蒲参胶囊中剂量组第7天可明显提高外周血bFGF和BDNF的蛋白水平。结论蒲参胶囊在一定程度上可提高大鼠脑缺血/再灌注后血清bFGF和BDNF的表达水平,改善神经再生微环境,进而促进神经功能的修复。

关键词(KeyWords)： 蒲参胶囊;脑缺血/再灌注;bFGF;BDNF

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 朱清,姚蓓蓓,吴明华

参考文献(References)：

• [1]Duehrkop C,Rieben R.Ischemia/reperfusion injury:effect of simultaneous inhibition of plasma cascade systems versus specific complement inhibition[J].Biochem Pharmacol,2014,88(1):12-22.
• [2]Menzies SA,Hoff JT,Betz AL.Middle cerebral artery occlusion in rats:a neurological and pathological evaluation of a reproducible model[J].Neurosurgery,1992,31(1):106-107.
• [3]施新猷.医用实验动物学[M].西安:陕西科学技术出版社,1989:382.
• [4]于潇,周鸿飞.中风病从血瘀论治探析[J].辽宁中医药大学学报,2013,24(11):252-253.
• [5]王立超,吴明华.通脑饮治疗急性脑梗死临床研究[J].中国中医急症,2015,24(11):1929-1931.
• [6]向诗余,曾强.活血化瘀药防治缺血性中风进展[J].中国中医急症,2002,11(3):213-214.
• [7]Ren JM,Finklestein SP.Time window of infarct reduction by intravenous basic fibroblast growth factor in focal cerebral ischemia[J].Eur J Pharmacol,1997,327(1):11-16.
• [8]Akimoto T,Hammerman MR.Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta[J].Am J Physiol Cell Physiol,2003,284(2):C371-377.
• [9]Wang Z,Zhang H,Xu X,et al.b FGF inhibits ER stress induced by ischemic oxidative injury via activation of the PI3K/Akt and ERK1/2 pathways[J].Toxicol Lett,2012,212(2):137-146.
• [10]Noshita T,Murayama N,Oka T,et al.Effect of b FGF on neuronal damage induced by sequential treatment of amyloidβand excitatory amino acid in vitro and in vivo[J].Eur J Pharmacol,2012,695(1-3):76-82.
• [11]Mamounas LA,Altar CA,Blue ME,et al.BDNF promotes the regenerative sprouting,but not survival,of injured serotonergic axons in the adult rat brain[J].J Neurosci,2000,20(2):771-782.
• [12]Yoshii A,Constantine-Paton M.Postsynaptic BDNF-Trk B signaling in synapse maturation,plasticity,and disease[J].Dev Neurobiol,2010,70(5):304-322.
• [13]Han BH,D'Costa A,Back SA,et al.BDNF blocks caspase-3activation in neonatal hypoxia-ischemia[J].Neurobiol Dis,2000,7(1):38-53.
• [14]Mattson MP,Lovell MA,Furukawa K,et al.Neurotrophic factors attenuate glutamate-induced accumulation of peroxides,elevation of intracellular Ca2+concentration,and neurotoxicity and increase antioxidant enzyme activities in hippocampal neurons[J].J Neurochem,1995,65(4):1740-1751.
• [15]Wu CL,Hwang CS,Yang DI.Protective effects of brain-derived neurotrophic factor against neurotoxicity of 3-nitropropionic acid in rat cortical neurons[J].Neurotoxicology,2009,30(4):718-726.