徐基杰,戴健,朱彦华,陈松,卓德华,李檫,周华

• 1:上海市宝山区中西医结合医院

摘要(Abstract)：

目的 研究鹿红方对缺血缺氧心肌细胞（CMs）抗凋亡能力的影响，并从SDF-1/CXCR4信号通路探讨其作用机制。方法 CMs分至对照组，缺血缺氧模型组以及鹿红方各剂量组。采用CCK-8法测定CMs凋亡情况以考查鹿红方对CMs抗凋亡能力的影响及量效果关系。后将CMs分至对照组、模型组、AMD3100组、鹿红方200μg/mL组以及鹿红方+AMD3100组。各组予相应干预措施后采用Western blotting检测Bax、Bcl-2、SDF-1和CXCR4蛋白表达。结果 CCK-8结果显示，鹿红方提高CMs抗凋亡能力具有量效关系。Western blotting结果显示：鹿红方200组与模型组比较，SDF-1和CXCR4的表达显著升高（P <0.001）,Bax表达显著降低，Bcl-2表达显著升高，Bax/Bcl-2值显著降低（P <0.001）；鹿红方+AMD3100组与鹿红方200组比较，Bax表达显著升高，Bcl-2表达显著降低，Bax/Bcl-2值显著升高（P <0.001）。结论 鹿红方可提高缺血缺氧状态下CMs的抗凋亡能力，其机制与上调SDF-1/CXCR4信号通路有关。

关键词(KeyWords)： 心肌细胞;凋亡;缺血缺氧;鹿红方;SDF-1/CXCR4信号通路

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（81904016）;; 上海市科学技术委员会医学创新研究专项面上项目（21Y11920100）

作者(Author): 徐基杰,戴健,朱彦华,陈松,卓德华,李檫,周华

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