水蛭素对大鼠实验性脑出血神经细胞凋亡及Bcl-2、Caspase-3表达影响的研究
殷萍,魏亚芬
摘要(Abstract)：
目的通过实验性大鼠脑出血模型研究水蛭素对脑出血后神经细胞凋亡的影响。方法以自体动脉血注入法建立实验性脑出血动物模型,而后干予组注入100 U水蛭素,出血组注入0.9%氯化钠注射液。于术后1、3、7 d处死动物。分别行HE染色、Bcl-2和Caspase-3免疫组化染色和TUNEL染色。结果干预组与出血组比较,血肿周围的细胞坏死和组织水肿明显减轻。Bcl-2表达升高,Caspase-3表达降低,TUNEL阳性细胞数均明显减少(P<0.05)。结论脑出血后局部应用水蛭素可以减轻脑出血后的组织水肿,抑制神经细胞凋亡,对血肿周围的神经组织具有保护作用。
关键词(KeyWords)： 脑出血;凋亡;水蛭素;Bcl-2;Caspase-3
基金项目(Foundation):
作者(Author): 殷萍,魏亚芬
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