茯苓四逆汤对脓毒症大鼠心肌保护机制的研究Study on Myocardial Protection Mechanism of Fuling Sini Decoction on Sepsis Rats
王评,彭晓洪,黄永莲,黄亚秀,李少萍,曹齐凤,陈冬杰,胡世平
摘要(Abstract)：
目的研究茯苓四逆汤对脓毒症大鼠心肌抑制的保护作用机制。方法取SPF级SD雄性大鼠100只,随机分成5组:假手术组、脓毒症组,茯苓四逆汤低剂、中、高剂量组(1.3 g/d、2.6 g/d、3.9 g/d)。假手术组、脓毒症组给予等容量0.9%氯化钠注射液,茯苓四逆汤各剂量组给予相应药物,均为灌胃给药;盲肠结扎穿孔法制作脓毒症模型,假手术组仅是暴露盲肠,造模后6 h开始给药,每日剂量分2次给药,直至造模后第3日,记录其生存状态,并在术前、术后24 h、48 h、72 h 3个时间点各选5只大鼠尾静脉取血并处死,取心肌组织后超低温保存备用,测定肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、心型脂肪酸结合蛋白(HFABP)水平。结果脓毒症组及中药各剂量组的大鼠TNF-α、IL-6、H-FABP明显升高,与假手术组差异有统计学意义(P <0.01);与脓毒症组同时间点比较,中药各剂量组的大鼠TNF-α、IL-6水平明显下降(P <0.01),H-FABP水平也明显下降(P <0.05);而中药各剂量组同时间比较发现,剂量越高,其TNF-α、IL-6水平下降越明显(P <0.01或P <0.05);不同剂量治疗组中比较发现,随着时间的延长,剂量越高的茯苓四逆汤对于改善H-FABP水平越好(P <0.05)。结论茯苓四逆汤对脓毒症大鼠心肌抑制具有良好保护作用,其机制可能与减少炎症释放有关。
关键词(KeyWords)： 脓毒症;茯苓四逆汤;心肌抑制;炎症因子;大鼠
基金项目(Foundation): 深圳市龙岗区医疗卫生科技计划项目(20170405191007276)
作者(Author): 王评,彭晓洪,黄永莲,黄亚秀,李少萍,曹齐凤,陈冬杰,胡世平
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