岑冰,王素莉,沈继春,葛海燕,丁悦

• 1:天津市武警后勤学院附属医院

摘要(Abstract)：

目的观察生脉注射液对糖尿病酮症酸中毒(DKA)家兔肾功能及MMP、TIMP蛋白的影响。方法制备DKA模型家兔,随机分为DKA组、盐酸二甲双胍组[85 mg/(kg·d)]及生脉注射液低[3 mg/(kg/d)]、中[9 mg/(kg·d)]、高[27 mg/(kg·d)]剂量组,对照组及DKA组给予等量0.9%氯化钠注射液灌胃。连续给药7 d后,检测各组家兔动脉血电解质、血气、血酮体、血清C反应蛋白(CRP)水平及肾功能,苏木素-伊红(HE)染色观察肾组织病变,蛋白印迹(WB)检测肾组织中MMP2、TIMP2、MMP9、TIMP1表达情况。结果与对照组比较,DKA组家兔表现为DKA症状,肾功能显著下降,肾组织出现纤维化、坏死、肾小管上皮变性等病变,血清CRP水平明显增高,肾组织TIMP1、TIMP2表达明显增加,MMP2、MMP9表达显著降低(均P <0.05)。与DKA组比较,生脉注射液低、中、高剂量组治疗后,家兔DKA症状减轻,肾功能明显恢复,肾组织病变程度显著改善,血清CRP水平显著下降,肾组织TIMP1、TIMP2表达明显降低,MMP2、MMP9表达显著升高,均呈剂量依赖效应(均P <0.05)。结论生脉注射液可保护DKA家兔肾功能,其机制可能通过抑制炎症反应、调节MMP和TIMP蛋白表达实现。

关键词(KeyWords)： 生脉注射液;糖尿病酮症酸中毒;肾功能;金属基质蛋白酶;组织金属蛋白酶抑制剂

Abstract:

Keywords:

基金项目(Foundation): 天津市卫计委科技基金攻关项目(2016KG107)

作者(Author): 岑冰,王素莉,沈继春,葛海燕,丁悦

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