俞坤强,李晓洁,彭洪卫,卓沛元,张颖铮,林晓敏,林如辉,陶静,陈立典

• 1:福建中医药大学
• 2:福建康复重点实验室
• 3:国家中医药管理局中医康复研究中心

摘要(Abstract)：

目的观察电针神庭、百会穴对局灶性脑缺血再灌注大鼠学习记忆的影响,并探讨其可能的机制。方法雄性Sprague-Dawley大鼠45只,采用线栓法制备局灶性脑缺血再灌注大鼠模型,造模成功后随机分为假手术组、模型组和电针组,各15只。电针组大鼠取神庭、百会穴,电针治疗7 d。各组大鼠在造模后第3天开始采用定位航行实验和空间探索实验检测学习记忆能力;观察各组大鼠神经行为学的表现;2,3,5-氯化三苯基四氮唑(TTC)检测脑梗死体积;苏木素-伊红(HE)染色观察海马区神经元破坏和水肿情况;蛋白免疫印迹(Western blotting)法检测大鼠左侧海马白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)炎症因子的表达。结果同模型组相比,电针组大鼠学习记忆能力改善(P<0.05),神经行为学评分降低(P<0.05),脑梗死体积减小(P<0.001),海马CA1区神经元破坏情况减轻,IL-1β、TNF-α炎症因子表达降低(P<0.05)。结论电针能改善局灶性脑缺血再灌注大鼠学习记忆能力,其机制可能与减轻脑内神经元的破坏和水肿、抑制IL-1β、TNF-α炎症因子表达相关。

关键词(KeyWords)： 脑缺血;电针;学习记忆;IL-1β;TNF-α

Abstract:

Keywords:

基金项目(Foundation): 科技部国际科技合作项目(2011DFG33240);; 福建省自然科学基金项目(2015J01335);; 福建省康复技术协同创新中心资助项目(X2012004-协同)

作者(Author): 俞坤强,李晓洁,彭洪卫,卓沛元,张颖铮,林晓敏,林如辉,陶静,陈立典

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