刘爽;徐家淳;孙伟明;曹艺萌;王以申;

• 1:天津中医药大学第二附属医院

摘要(Abstract)：

目的 观察补肾法中药通过调控ERK/MAPK信号通路关键靶点蛋白，探讨其对Aβ25-35诱导的阿尔茨海默病（AD）大鼠模型的神经保护作用机制。方法 取36只雌性SD大鼠随机分为假手术组、模型组、中药组，每组12只。采用Aβ25-35侧脑室注入建立AD大鼠模型，中药组予补肾中药，模型组及假手术组予蒸馏水灌胃，观察各组大鼠认知记忆能力、海马神经元凋亡、p-ERK1/2蛋白表达情况。结果 1)AD认知记忆功能检测：与模型组相比，中药组大鼠定位航行实验显示，平均速度无显著差异（P> 0.05），潜伏时间明显缩短，学习能力明显上升，差异具有统计学意义（P <0.05）。2)AD海马神经元凋亡检测：光镜下观察尼氏染色结果显示，与模型组相比，中药组神经元凋亡数量明显减少（P <0.01）。3)ERK/MAPK信号通路关键靶点p-ERK1/2蛋白表达的检测：Western blotting检测结果显示，与假手术组相比，模型组p-ERK1/2蛋白的表达均明显降低，差异具有统计学意义（P <0.05）；与模型组相比，中药组p-ERK1/2蛋白的表达明显增加，差异具有统计学意义（P <0.05）。结论 补肾法中药对Aβ25-35诱导的AD模型大鼠海马神经元损伤具有保护作用，可明显改善AD模型大鼠认知和记忆能力，减少海马神经元的凋亡，其作用机制可能调控p-ERK1/2蛋白表达，激活ERK/MAPK信号通路有关。

关键词(KeyWords)： 老年性痴呆;阿尔茨海默病;补肾法;ERK/MAPK信号通路;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上基金项目（81973797）;; 天津市教委科研计划项目（2021ZD023）

作者(Authors): 刘爽;徐家淳;孙伟明;曹艺萌;王以申;

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