具栖冬青苷对脑缺血再灌注损伤大鼠神经功能影响及其机制研究Effect and Mechanism of Pedunculoside on Behavior of Rats with Cerebral Ischemia-reperfusion Injury
陈皓;苏祖禄;王珂厶;余作琼;
摘要(Abstract)：
目的 观察具栖冬青苷对脑缺血再灌注损伤大鼠神经元的保护作用及其机制。方法 60只健康雄性Wistar大鼠随机分为6组：假手术组，模型组，Caspase-3抑制剂组（4 mg/kg），具栖冬青苷高（200 mg/kg）、中（100 mg/kg）、低剂量组（50 mg/kg），每组10只。各组于造模前连续灌胃相应药物7 d，假手术组和模型组给予生理盐水。MCAO法制造大鼠脑缺血模型，2 h后拔栓，再灌注24 h。对各组大鼠神经功能进行评分；水迷宫法和跳台法检测大鼠学习记忆能力；流式细胞仪检测神经元凋亡情况；荧光定量PCR和Western blotting法检测凋亡相关Caspase-3、Bcl-2、Bax m RNA和蛋白的表达。结果 与模型组相比，具栖冬青苷高、中、低剂量组大鼠的神经功能评分显著降低（P <0.05）；水迷宫潜伏期缩短，穿越安全平台次数增多（P <0.05）；跳台潜伏期延长，错误次数降低（P <0.05）；神经元凋亡率显著降低（P <0.05）;Caspase-3、Bax的mRNA和蛋白表达显著降低（P <0.05）,Bcl-2的表达显著升高（P <0.05）。结论 具栖冬青苷能显著改善脑缺血再灌注大鼠的神经行为学结果，其机制可能是通过抑制凋亡通路BAX/Caspase-3而发挥抗神经元凋亡作用。
关键词(KeyWords)： 脑缺血再灌注;神经元凋亡;具栖冬青苷;Caspase-3;Bcl-2;Bax;大鼠
基金项目(Foundation): 重庆市科委基础与前沿立项（cstc2018jcyjAX0821）;; 重庆医药高等专科学校校级课题（ygz2020208）
作者(Authors): 陈皓;苏祖禄;王珂厶;余作琼;
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