宋晓龙,宋峻,潘仁友,王蕾,孙伟新,袁春玲

• 1:南京中医药大学盐城附属医院江苏省盐城市中医院
• 2:徐州医科大学

摘要(Abstract)：

目的 研究利心水方对大鼠心肌纤维化的影响。方法 应用腹主动脉缩窄法制备大鼠心肌纤维化模型，用低、中、高剂量利心水方干预治疗5周后，超声动图检测不同处理组中大鼠的心肌功能，通过HE染色法分析不同处理组中大鼠心肌组织的变化，应用Masson染色对比不同组大鼠的胶原蛋白的含量，利用RT-q PCR和Western blotting对比不同处理组中大鼠心肌组织中CollagenⅠ、α-SMA、TGF-β、Fibronectin的表达。结果与假手术组相比，模型组中大鼠的LVEDD和LVESD明显增加，FS和EF较假手术组明显降低（P <0.01），低、中、高剂量的利心水方组大鼠LVEDD和LVESD较模型组显著下降，高剂量组效果最显著，而利心水方组大鼠FS和EF较模型组显著升高（P <0.05）。HE和Masson染色显示：与假手术组对比，模型组中大鼠心肌细胞排列紊乱，细胞肥大，细胞间隙明显增大，并可见大量胶原的沉积；与模型组相比，利心水方组大鼠心肌细胞排列较为整齐，细胞间隙较小，有少量胶原组织的沉积。RT-qPCR和Western blotting结果显示，与假手术组相比，模型组大鼠心肌组织的CollagenⅠ、α-SMA、TGF-β、Fibronectin mRNA和蛋白的表达水平显著升高（P <0.01），利心水方组干预后，心肌组织的CollagenⅠ、α-SMA、TGF-β、Fibronectin mRNA及其蛋白的表达水平均显著下降（P <0.05）。结论 中药复方利心水方对于大鼠的心肌纤维化有一定的保护作用。

关键词(KeyWords)： 心肌纤维化;心肌组织;利心水方;大鼠

Abstract:

Keywords:

基金项目(Foundation): 江苏省中医药科技发展计划项目（YB201968）

作者(Author): 宋晓龙,宋峻,潘仁友,王蕾,孙伟新,袁春玲

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