周文佳;尚云峰;朱文新;杨卓;卢雪笛;申楠;向黎黎;陆小龙;熊辉;

• 1:湖南省岳阳市中心医院
• 2:湖南省常德市第一中医院
• 3:湖南中医药大学第二附属医院
• 4:湖南中医药大学

摘要(Abstract)：

目的 分离培养大鼠骨髓间充质干细胞（BMSCs），运用桃红四物汤含药血清和Hippo信号通路抑制剂Verteporfin干预，进而观察BMSCs增殖和成骨分化的能力。方法 采用全骨髓贴壁法体外培养BMSCs，选用10倍等效剂量的桃红四物汤灌服大鼠制备桃红四物汤含药血清，采取0.9%氯化钠注射液灌胃大鼠制取空白组对照血清，将BMSCs分为3组：空白血清组、桃红四物汤含药血清组（THSWT组）以及桃红四物汤含药血清+Verteporfin组（THSWT+V组），使用CCK-8分析法分析4个时相点0、1、3、7 d细胞增殖活力；干预7 d后，进行ALP测定，观察骨向分化程度；处理21 d后，进行茜素红染色，观察钙积累，Western blotting法检测各组细胞成骨关键因子及Hippo通路关键蛋白表达。结果 THSWT组BMSCs细胞增殖能力明显高于THSWT+V组及空白血清组（P <0.01）;THSWT组BMSCs成骨分化能力及钙盐沉积程度高于THSWT+V组及空白血清组；THSWT组中YAP蛋白表达低于空白血清组、TAZ蛋白表达高于空白血清组（P <0.05）;THSWT组ALP、OCN、RUNX2、Osterix等成骨关键因子蛋白表达明显高于THSWT+V组及空白血清组（P <0.05）。结论 桃红四物汤可促进BMSCs的增殖和成骨分化能力，从而促进骨折愈合，其作用机制为调节Hippo-YAP信号通路，激活其下游关键节点YAP蛋白及TAZ蛋白，诱导BMSCs成骨分化基因转录。

关键词(KeyWords)： 骨折;桃红四物汤;BMSCs;Hippo-YAP信号通路;成骨分化;大鼠

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（81874478）;; 湖南省教育厅优秀青年项目（19B420）

作者(Authors): 周文佳;尚云峰;朱文新;杨卓;卢雪笛;申楠;向黎黎;陆小龙;熊辉;

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