余希婧;黄辉;胡秀武;朱俐娜;耿乐乐;吕明芳;华水生;

• 1:江西省南昌市洪都中医院
• 2:江西省南昌市长蛇灸效应机制和督脉特异性重点实验室江西省针灸医学临床研究中心

摘要(Abstract)：

目的 探讨长蛇灸通过激活过氧化物酶体增殖激活受体（PPARγ）来抑制炎性细胞因子表达从而缓解强直性脊柱炎（AS）的机制。方法 以3月龄大鼠为模型，用人蛋白多糖提取物溶于二甲基十八胺2 mg腹腔注射，每隔2周注射1次，共4次，构建强直性脊柱炎模型。在模型建立初期开始治疗干预，即用长蛇灸治疗2周，同时评估AS大鼠模型的严重程度。治疗结束后，采用ELISA法检测炎症细胞因子如白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-17(IL-17)和白细胞介素-23(IL-23)蛋白变化，qPCR检测IL-6、TNF-α、IL-1β、IL-17和IL-23的m RNA变化，Western blotting法检测PPARγ激活程度。结果 在AS初期即用长蛇灸治疗可显著下调AS大鼠外周病进展评分（P <0.05），且可显著抑制外周血TNF-α、IL-1、IL-17、IL-23和IL-6的蛋白和m RNA表达水平（P <0.05），同时激活组织中PPARγ的表达（P <0.05）。结论 在AS大鼠初期采用长蛇灸治疗可能通过激活PPARγ来抑制IL-6、IL-1β、TNF-α、IL-17和IL-23的表达，最终缓解强直性脊柱炎。

关键词(KeyWords)： 强直性脊柱炎;长蛇灸;PPARγ;炎症因子;大鼠

Abstract:

Keywords:

基金项目(Foundation): 江西省重点研发计划项目（20202BBGL73002）

作者(Authors): 余希婧;黄辉;胡秀武;朱俐娜;耿乐乐;吕明芳;华水生;

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